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LEAF™ Purified anti-mouse LIF Antibody

Pricing & Availability
Clone
Poly5232 (See other available formats)
Regulatory Status
RUO
Other Names
Leukemia Inhibitory factor, HILDA, CDF, DIA, MLPLI
Isotype
Goat Polyclonal IgG
Ave. Rating
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Product Citations
publications
Poly5232_-LEAF_-LIF_Neut_042023
Recombinant mouse LIF (Cat. No. 554002) (black circles) inhibits proliferation of M1 cells. LEAF™ purified anti-mouse LIF (clone Poly5232) (purple squares) neutralizes the cell proliferation inhibited by recombinant mouse LIF at 0.5 ng/mL on M1 cells in a dose-dependent manner, whereas the LEAF™ purified goat IgG, isotype control (gray triangles) does not have the effect. ND50 range: 0.004 – 0.028 µg/mL
  • Poly5232_-LEAF_-LIF_Neut_042023
    Recombinant mouse LIF (Cat. No. 554002) (black circles) inhibits proliferation of M1 cells. LEAF™ purified anti-mouse LIF (clone Poly5232) (purple squares) neutralizes the cell proliferation inhibited by recombinant mouse LIF at 0.5 ng/mL on M1 cells in a dose-dependent manner, whereas the LEAF™ purified goat IgG, isotype control (gray triangles) does not have the effect. ND50 range: 0.004 – 0.028 µg/mL
  • Poly5232_-LEAF_-LIF_WB_042023
    Whole cell extracts (15 µg total protein) from Raw264.7 cells spiked with the indicated amount of recombinant mouse LIF protein (Cat. No. 554008) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL LEAF™ purified anti-mouse LIF (clone Poly5232) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP-conjugated donkey anti-goat. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
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523205 100 µg 400 CHF
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Description

Leukemia inhibitory factor (LIF) is a 20 kD protein that belongs to the IL-6 receptor family. It binds to a heterodimeric receptor composed of a type I transmembrane ligand-binding subunit, the LIF-specific subunit gp190 (LIFR), and the type I transmembrane signal transducing subunit gp130, shared with the other members of the IL-6 family. It especially signals through STAT3 and JAK kinases. LIF expression has been observed in various tissues including thymus, lung, and neuronal tissues. LIF displays diverse biological effects, but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contribute to stem cell self-renewal. LIF can be upregulated by proinflammatory cytokines such as TNF-α and IL-17, and elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis. Human and mouse LIF share 79% sequence homology and exhibit cross-species activity. However, LIF inhibition of stem cell differentiation appears to be mouse-specific. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Recombinant Mouse LIF
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.
Preparation
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Upon receipt, store frozen at -20°C. Make small volume aliquots if needed and avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
Application

Neut - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by neutralization of proliferation inhibited by recombinant mouse LIF (Cat. No. 554002) at 0.5 ng/mL on M1 cells. ND50 range: 0.004 – 0.028 µg/mL. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2941596 (BioLegend Cat. No. 523205)

Antigen Details

Structure
Monomer; mouse LIF is a 203 amino acid protein with a predicted molecular weight of approximately 22 kD.
Distribution

Expressed in the trophectoderm of the developing embryo, activated CD4+ T cells

Function
Inhibits embryonic stem cell differentiation, induces the terminal differentiation of myeloid leukemic cells
Interaction
Embryonic stem cells
Ligand/Receptor
LIFR
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors
Biology Area
Cell Biology, Immunology, Stem Cells
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References
  1. Moreau JF, et al. 1988. Nature. 336:690-2.
  2. Tomida M, et al. 1993. FEBS Lett. 334:193-7.
  3. Metcalfe SM. 2011. Genes Immun. 12:157-68.
  4. Voyle RB, et al. 1999. Exp Cell Res. 249:199-211.
  5. Slaets H, et al. 2010. Trends Mol Med. 16:493-500.
Gene ID
16878 View all products for this Gene ID
UniProt
View information about LIF on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

View All LIF Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse LIF Poly5232 ELISA Capture
Biotin anti-mouse LIF Poly5232 ELISA Detection
LEAF™ Purified anti-mouse LIF Poly5232 Neut,WB
Go To Top Version: 1    Revision Date: 04.21.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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