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LEAF™ Purified anti-rat IL-13 Antibody

Pricing & Availability
Clone
Poly23A06 (See other available formats)
Regulatory Status
RUO
Other Names
IL-13
Isotype
Goat Polyclonal IgG
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Product Citations
publications
Poly23A06_LEAF_Neut_072626.
Recombinant rat IL-13 (Cat. No. 751702) (black circles) induces proliferation of TF-1 cells. LEAF™ purified anti-rat IL-13 (clone Poly23A06) (purple squares) neutralizes the TF-1 cell proliferation induced by recombinant rat IL-13 at 7 ng/mL in a dose-dependent manner, whereas the LEAF™ purified goat IgG isotype control (gray triangles) does not have the effect. ND50 range is 0.04-0.24 µg/mL.
  • Poly23A06_LEAF_Neut_072626.
    Recombinant rat IL-13 (Cat. No. 751702) (black circles) induces proliferation of TF-1 cells. LEAF™ purified anti-rat IL-13 (clone Poly23A06) (purple squares) neutralizes the TF-1 cell proliferation induced by recombinant rat IL-13 at 7 ng/mL in a dose-dependent manner, whereas the LEAF™ purified goat IgG isotype control (gray triangles) does not have the effect. ND50 range is 0.04-0.24 µg/mL.
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532604 100 µg 470 CHF
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Description

Rat IL-13 was initially cloned from the rat renal cortex RNA after the induction of anti-glomerular basement membrane antibody-induced glomerulonephritis. Rat IL-13 respectively shows 63% and 79% homology with the human and mouse proteins at the amino acid level. IL-13 is an immunoregulatory cytokine secreted predominantly by activated Th2 cells, and is a key mediator in the pathogenesis of allergic inflammation. IL-13 shares many functional properties with IL-4, and they share a common receptor subunit: the alpha subunit of the IL-4 receptor (IL-4Rα). IL-13 mediates its effects by interacting with a complex receptor system comprised of IL-4Rα and two IL-13 binding proteins: IL-13Rα1 and IL-13Rα2. The ligation of the IL-13 receptor complex results in signaling via the insulin receptor substrate (IRS)-1 and 2 and STAT-6 pathways. IL-13, like IL-4, is a cytokine produced by Th2 cells in response to the signaling through the T cell antigen receptor and by mast cells and basophils upon cross-linkage of the high-affinity receptor for immunoglobulin E (IgE). IL-13 has been implicated in airway hypersensitivity and mucus hypersecretion, chronic obstructive pulmonary disease (COPD), inflammatory bowel disease, and parasitic nematode expulsion.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rat
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Recombinant Rat IL-13
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.1 EU/μg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test
Preparation
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Upon receipt, store frozen at -20°C. Make small volume aliquots if needed and avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by neutralizing proliferation induced by recombinant rat IL-13 (Cat. No. 751702) at 7 ng/mL in TF-1 cells. ND50: 0.04 - 0.24 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Monomer
Distribution

IL-13 is secreted by activated T cells, Th2 cells, basophils, mast cells, activated eosinophils, natural killer cells (NK), and macrophages.

Function
IL-13 modulates human monocytes, macrophages, and B cells. IL-13 induces an increase of MHC class II expression in human monocytes and macrophages, and a direct inhibition of inflammatory cytokines such as TNF-α and IL-1β
Interaction
IL-13 receptors are expressed on human B cells, basophils, eosinophils, mast cells, endothelial cells, fibroblasts, monocytes, macrophages, respiratory epithelial cells, and smooth muscle cells.
Ligand/Receptor
IL-4α, IL-13Rα1, and IL-13Rα2
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Lakkis FG and Cruet EN. 1993. Biochem Biophys Res Commun. 197:612-8.
  2. Papasavvas E, et al. 2005. J Immunol. 175:5532-40.
  3. Kelly-Welch A, et al. 2005. Sci STKE. 293:cm8.
  4. Hershey GK. 2003. J Allergy Clin Immunol. 111:677-90.
  5. Harris J, et al. 2007. Immunity. 27:505-17.
  6. LaPorte SL, et al. 2008. Cell. 132:259-72.
  7. Brightling CE,et al. 2010. Clin Exp Allergy. 40:42-9.
  8. De Paiva CS, et al. 2011. Mucosal Immunol. 4:397-408.

 

Gene ID
116553 View all products for this Gene ID
UniProt
View information about IL-13 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 07.26.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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