LEGEND MAX™ High Sensitivity Human IL-10 ELISA Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
Cytokine synthesis inhibitory factor (CSIF), T-cell growth inhibitory factor (TGIF), GVHDS
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Product Citations
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a.
High-Sensitivity_Human-IL-10_1_ELISA-kit
Representative sandwich immunoassay standard curve generated using serial dilutions of LEGEND MAX™ Human IL-10 Standard ranging from 0.625 pg/mL to 40 pg/mL.
  • a.
High-Sensitivity_Human-IL-10_1_ELISA-kit
    Representative sandwich immunoassay standard curve generated using serial dilutions of LEGEND MAX™ Human IL-10 Standard ranging from 0.625 pg/mL to 40 pg/mL.
  • b.
High-Sensitivity_Human-IL-10_2_ELISA-kit
    Percentage of samples containing low concentrations of IL-10 that were detected using either a conventional IL-10 ELISA or the LEGEND MAX™ High Sensitivity Human IL-10 ELISA Kit (Cat. No. 430617).
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430617 1 Pre-coated Plate 540 CHF
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Description

IL-10 (Interleukin 10, B cell derived T cell growth factor, B-TCGF, Cytokine synthesis inhibitory factor CSIF, T-cell growth inhibitory factor, TGIF) was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) due to its ability to inhibit cytokine production by TH1 clones. IL-10 is expressed in activated CD8+ and CD4+ T cells, activated monocytes, mast cells, and Ly-1 B (in human cells). IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI that allows the virus to suppress the immune response. The functions of IL-10 include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the TH2 cell response. 

The BioLegend LEGEND MAX™ High Sensitivity Human IL-10 ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a monoclonal mouse anti-human IL-10 capture antibody. The detection antibody is a biotinylated monoclonal mouse anti-human IL-10 antibody. After washing away any unbound biotinylated detection antibody, a streptavidin-polymer HRP is used for detection. Streptavidin-Polymer HRP enhances the sensitivity of the kit, allowing for detection of low concentrations of IL-10. This kit is specifically designed for the accurate quantitation of human IL-10 in serum, citrate plasma, heparin plasma, cell culture supernatant, and urine. This kit is analytically validated with ready-to-use reagents.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Human IL-10 Pre-coated 96-well Strip Microplate 
  • Human IL-10 Detection Antibody (200X)
  • Human IL-10 Lyophilized Standard
  • Streptavidin-Polymer HRP
  • Assay Buffer A
  • 20X Wash Buffer
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Verified Reactivity
Human
Application

ELISA

Sensitivity
0.171 ± 0.068 pg/mL
Standard Range
0.625 – 40 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Microplate shaker
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Polypropylene vials

Related FAQs

In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

Go To Top Version: 1    Revision Date: 10.03.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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