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LEGEND MAX™ Mouse OVA Specific IgE ELISA Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
OVA-IgE Precoated ELISA Kit
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Product Citations
publications
OVA-IGE_Mouse_LegMax_ELISA_083112
  • OVA-IGE_Mouse_LegMax_ELISA_083112
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439807 1 Pre-coated Plate 462 CHF
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Description

Immunoglobulin E has long been regarded as a major molecular component of atopic diseases, including allergy and asthma. Asthma is a chronic respiratory disease characterized by bronchial inflammation and airway hyperresponsiveness. Clinical studies have found a close association between asthma and serum IgE levels. Allergen-specific IgE has been detected in nasal mucosa from allergic patient. Examination of murine models of human allergic asthma has provided novel insight into the pathobiologic events linking airway inflammation with airway dysfunction.

Ovalbumin (OVA) is the main protein found in egg white, making up 60-65% of the total protein. Ovalbumin is an important protein in several different areas of research and commonly used to stimulate an allergic reaction in mice (e.g. established model allergen for airway hyperresponsiveness).

The BioLegend LEGEND MAX™ Mouse OVA specific IgE ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with OVA. This kit is specifically designed for the accurate quantitation of mouse OVA specific IgE from cell culture supernatant, serum, plasma, and other biological fluids. It is analytically validated with ready-to-use reagents.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • OVA Pre-coated 96-well Strip Microplate
  • Mouse IgE Dectection Antibody
  • Mouse OVA specific IgE Standard
  • Matrix C (for serum and plasma samples only)
  • Avidin-HRP D
  • Assay Buffer A
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Verified Reactivity
Mouse
Application

ELISA
Application References

(PubMed link indicates BioLegend citation)
  1. Miller M, et al. 2014. J. Immunol. 192:3475. PubMed
  2. Nau F, et al. 2015. Am J Physiol Lung Cell Mol Physiol. 308:191. PubMed
Product Citations
  1. Nguyen TV, et al. 2022. Int J Mol Sci. 23: . PubMed
  2. Lee H, et al. 2017. Exp Lung Res. 10.1080/01902148.2017.1339141. PubMed
  3. Zhou J, et al. 2021. Environ Toxicol. 36:177. PubMed
  4. Cho S, et al. 2016. J Exp Med. 213: 235 - 249. PubMed
  5. Seo YS, et al. 2019. Sci Rep. 9:2312. PubMed
  6. Woo YD, et al. 2020. J Allergy Clin Immunol. . PubMed
  7. Nakajima T, et al. 2021. J Exp Med. 218:. PubMed
  8. Le H, et al. 2017. Biochem Biophys Res Commun. 10.1016/j.bbrc.2017.06.038. PubMed
  9. Fakih D, et al. 2015. Am J Physiol Lung Cell Mol Physiol. 309: L1333 - L1343. PubMed
  10. Miller M, et al. 2014. J Immunol. 192:3475. PubMed
  11. Xu H, et al. 2019. Exp Ther Med. 1.278472222. PubMed
  12. Li C, et al. 2016. PLoS One. 11: 0161164. PubMed
  13. Nau F, et al. 2015. Am J Physiol Lung Cell Mol Physiol. 308:191. PubMed
  14. Ren J, et al. 2015. PLoS One. 10: e0140018. PubMed
  15. Ding FX, et al. 2018. Pediatr Res. 84:125. PubMed
  16. Li X, et al. 2020. Open Med (Wars). 15:483. PubMed
Sensitivity
20.7 pg/mL
Standard Range
0.156-10 ng/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Related FAQs

In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

Go To Top Version: 2    Revision Date: 03.11.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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