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LEGENDplex™ Human Inflammation Panel 1 (13-plex) with V-bottom Plate

Pricing & Availability
Regulatory Status
RUO
Other Names
IL-1β, IFN-α2,IFN-γ, TNF-α, MCP-1, IL-6, CXCL8 (IL-8), IL-10, IL-12p70, IL-17A, IL-18, IL-23, IL-33
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Product Citations
publications
Cat # Size Price Quantity Save
740809 100 tests 1761 CHF
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Description

Inflammation is a biological response triggered by exposure to harmful stimuli such as tissue damage or foreign antigens. This response is initiated by resident immune cells, and then mediated through the actions of various cytokines and chemokines. Abnormalities in inflammation also underlie a large variety of chronic human diseases. Accurate measurement of inflammatory mediators is therefore critical to further our understanding of the role these molecules play in shaping the immune response to injury or infection.

The Human Inflammation Panel 1 is a bead-based multiplex assay, using fluorescence–encoded beads suitable for use on various flow cytometers. This panel allows simultaneous quantification of 13 human inflammatory cytokines/chemokines, including IL-1β, IFN-α2, IFN-γ, TNF-α, MCP-1 (CCL2), IL-6, IL-8 (CXCL8), IL-10, IL-12p70, IL-17A, IL-18, IL-23, and IL-33. This assay panel provides higher detection sensitivitiy and broader dynamic range than traditional ELISA methods. The panel has been validated for use on serum and cell culture supernatant samples.

The Human Inflammation Panel 1 is designed to allow flexible customization within the panel. Please visit www.biolegend.com/legendplex for more information on how to mix and match within the panel.

This assay is for research use only.

Product Details
View Current Manual (pdf)

Kit Contents

Kit Contents
  • Setup Beads: PE Beads 
  • Setup Beads: Raw Beads 
  • Capture Beads
  • Detection Antibody 
  • Standard Cocktail, Lyophilized 
  • SA-PE 
  • Matrix B3, Lyophilized 
  • Assay Buffer 
  • Wash Buffer, 20X 
  • V-bottom Plate 
  • Plate Sealers 

Please note: Lots manufactured before September 2021 may include a different version of the “Matrix B.” There is no change in the protocol. Simply use the Matrix reagent you received as suggested in the current manual.

Product Details

Verified Reactivity
Human
Application

Multiplex
Learn more about LEGENDplex™ at biolegend.com/legendplex

Download the LEGENDplex™ software here.
Product Citations
  1. Hühn D, et al. 2022. Mol Oncol. 16:148. PubMed
  2. Ogishi M, et al. 2023. J Exp Med. 220: . PubMed
  3. Rössler T, et al. 2022. Metabolites. 12: . PubMed
  4. Nadkarni R, et al. 2022. J Exp Med. 219:. PubMed
  5. Duhalde Vega M, et al. 2022. Sci Adv. 8:eabn6545. PubMed
  6. Bozzini S, et al. 2023. Biomed Pharmacother. 162:114640. PubMed
  7. Raparelli V, et al. 2023. Clin Res Cardiol. . PubMed
  8. Paunovic V, et al. 2023. Cells. 12:. PubMed
  9. Berezhnoy G, et al. 2023. Front Immunol. 14:1144224. PubMed
  10. Serrano-Macia M, et al. 2022. Cell Death Discov. 8:316. PubMed
  11. Sampurno S, et al. 2022. Ann Surg Oncol. :. PubMed
  12. Koutsakos M, et al. 2021. Cell Reports Medicine. 2(3):100208. PubMed
  13. Ruchakorn N, et al. 2019. Arthritis Res Ther. 21:287. PubMed
  14. Pace F, et al. 2021. Microbiol Spectr. 9:e0136921. PubMed
  15. Wangriatisak K, et al. 2022. Lupus Sci Med. 9:. PubMed
  16. Zaal A, et al. 2020. Front Immunol. 11:305. PubMed
  17. Nguyen THO, et al. 2021. Immunity. 54:1066. PubMed
  18. Lee CQE, et al. 2021. Nat Commun. 12:2130. PubMed
  19. Krueger A, et al. 2022. Front Microbiol. 12:789042. PubMed
  20. Stanczak MA, et al. 2021. Nat Commun. 12:2133. PubMed
  21. Cichon MA, et al. 2022. Elife. 11:. PubMed
  22. Moreira AC, et al. 2021. Viruses. 13:. PubMed
  23. Chen Y, et al. 2021. Front Immunol. 12:623610. PubMed
  24. He X, et al. 2020. J Infect Dis. 222:1550. PubMed
  25. Winheim E, et al. 2021. PLoS Pathog. 17:e1009742. PubMed
  26. Unterberg M, et al. 2022. BMC Anesthesiol. 22:12. PubMed
  27. Cao X, et al. 2020. Curr Protoc Stem Cell Biol. 52:e108. PubMed
  28. Sananez I, et al. 2021. EBioMedicine. 72:103615. PubMed
Materials Not Included
  • Flow Cytometer
  • Pipettes and Tips
  • Reagent Reservoirs for Multichannel Pipettes
  • Polypropylene Microfuge Tubes, in 96-Tube Rack
  • Vortex Mixer
  • Sonicator
  • Aluminum foil
  • Absorbent pads
  • Plate Shaker
  • Centrifuges
  • A Vacuum Filtration Unit and a Vacuum Source (if using filter plates)
  • Manual

Related FAQs

If I don't have a vacuum, how do I remove the liquid from my plate?

If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.

Should I perform the assay with the filter plates or with V-bottom plates?

Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.

After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?

The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.

What is the shelf life of LEGENDplex™ kits?

LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.

Is special software required for data analysis?

Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.

Go To Top Version: 5    Revision Date: 06.23.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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