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PBMCs were prepared and stained with CD19 (clone HIB19) PE. PE-positive cells were then isolated using MojoSort™ Human anti-PE Nanobeads. Dead cells were excluded by 7-AAD. -
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PBMCs were prepared and stained with TCR Vγ9 (clone B3) PE. PE-positive cells were then isolated using MojoSort™ Human anti-PE Nanobeads. Dead cells were excluded by 7-AAD. -
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PBMCs were prepared and stained with CD3 (clone UCHT1) PE. PE-positive cells were then isolated using MojoSort™ Human anti-PE Nanobeads. Dead cells were excluded by 7-AAD. -
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 480091 | 100 µL | 225 CHF | ||||
| 480092 | 1 mL | 660 CHF | ||||
Human PE labeled PBMCs are either selected or depleted in a two-step separation process. The cells are first incubated with a BioLegend anti-human PE-conjugated antibody, followed by incubating with directly conjugated anti-PE Nanobeads. The PE+, magnetically labeled fraction is retained by the use of a magnetic separator. After collection or depletion of the PE+ cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.
MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.
Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage.
Kit Contents
- Kit Contents
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For Cat# 480091:
- 1 vial containing 100 µL of Human TruStain FcX™ (Fc Receptor Blocking Solution)
- 1 vial containing 100 µL of Human anti-PE Nanobeads
For Cat# 480092:
- 1 vial containing 1 ml of Human TruStain FcX™ (Fc Receptor Blocking Solution)
- 1 vial containing 1 ml of Human anti-PE Nanobeads
Product Details
- Verified Reactivity
- Human
- Formulation
- Aqueous solution containing 0.05% sodium azide and 0.3% BSA.
- Preparation
- Conjugated nanobeads: Protein-coated magnetic beads.
- Storage & Handling
- All components should be stored undiluted between 2°C and 8°C.
- Application
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Cell Separation (MojoSort™) - Quality tested
- Recommended Usage
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Volume of MojoSort™ Human anti-PE Nanobeads should be adjusted depending on starting percentage of cell type to be isolated. For 1x107 cells in 100 µl of buffer, use the following volumes:
Cell type
Cell Frequency in PBMCs
Volume of Nanobeads (for 1x107 cells in 100 µl of buffer)
CD3
50-60%
20 µl
CD4 (including monocytes)
50-55%
20 µl
CD8a (including low expressing cells)
15-20%
10 µl
CD19
5-10%
4 µl
TCR Vγ9
1-3%
2 µl
Always use 5 µL Human TruStain for 1x107 cells in 100 µl of buffer. If additional vials of TruStain are required, please order Cat: 422301 or 422302.
For low frequency cells, pre-dilute the Nanobeads in order to pipette a minimum of 5 µl of any solution. For example, to isolate TCR Vγ9+ cells, pre-dilute 10 µl of PE Nanobeads in 40 µl of MojoSort™ buffer and add 10 µl of that dilution per sample. Avoid working with small volumes.
- Application Notes
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The MojoSort™ Human anti-PE Nanobeads are designed to be used with BioLegend PE (phycoerythrin) conjugated antibodies against human cells. It can be used as a two-step kit for the positive selection or depletion of PE+ labeled cells from peripheral blood mononuclear cells (PBMCs).
Nanobead dilution to use in column: To be optimized by end users - Product Citations
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Antigen Details
- Gene ID
- NA
- UniProt
- View information about PE on UniProt.org
Related FAQs
- Is there a way to detach your magnetic particles from the cell surface?
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MojoSort™ Nanobeads: These magnetic particles cannot be removed from cells. This includes standalone Nanobeads and MojoSort kits using Nanobeads. We have found that cells are functional without the need to detach the magnetic Nanobeads.
MojoSort Microbeads: These magnetic particles may be released following the detailed product-specific protocol.
- What is the size of your magnetic particles?
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MojoSort Nanobeads: the average diameter is approximately 130 nm.
MojoSort Microbeads: the average diameter is approximately 1 μm.
- Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?
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MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems. Please contact BioLegend Technical Service for more product-specific information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.
- What antibodies are present in the depletion cocktails provided for isolation kits?
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Please contact our technical service team for further assistance.
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