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PBMCs were prepared to deplete CD14+ cells followed by CD4 selection by using the MojoSort™ Human CD4 T cell Selection Kit. Cells were stained with CD4 APC (clone RPA-T4). Dead cells were excluded by 7-AAD.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 480038 | 200 tests | 872 CHF | ||||
In this two-step kit, monocytes are first depleted by incubating PBMCs with biotin anti-human CD14 antibody, followed by Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator; the unlabeled fraction is collected in a clean tube. In the second step, CD4+ T cells are positively selected from the previous unlabeled fraction by incubating it with directly conjugated Human CD4 Nanobeads. The second magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD4+ T cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.
MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.
Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage.
Kit Contents
- Kit Contents
-
- 1 vial containing 1 ml of biotin anti-human CD14 antibody
- 2 vials containing 1 ml each of Streptavidin Nanobeads
- 2 vials containing 1 ml each of Human CD4 Nanobeads
Product Details
- Verified Reactivity
- Human
- Formulation
-
Antibody: Phosphate-buffered solution containing 0.09% sodium azide, pH 7.2 with stabilizer.
Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide. - Preparation
-
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Conjugated particles: protein-coated magnetic beads. - Storage & Handling
- All components should be stored undiluted between 2°C and 8°C.
- Application
-
Cell Separation (MojoSort™) - Quality tested
- Recommended Usage
-
5 µl of biotin anti-human CD14 antibody for 1x107 cells in 100 µl of buffer.
10 µl Streptavidin Nanobeads for 1x107 cells in 100 µl of buffer.
10 µl Human CD4 Nanobeads for 1 x 107 cells in 100 µl of buffer. - Application Notes
-
This is a two-step kit designed for the positive selection of human CD4+ T cells from peripheral blood mononuclear cells (PBMCs), after the depletion of CD14+ cells.
Each lot has been individually optimized. Do not mix and match components from different lots or different kits.To find out the dilution to use this product in separation columns please download the CoA.
- Additional Product Notes
-
View more applications data for this product in our Scientific Poster Library.
Antigen Details
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Gene ID
- NA
- UniProt
- View information about CD4 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- Is there a way to detach your magnetic particles from the cell surface?
-
MojoSort™ Nanobeads: These magnetic particles cannot be removed from cells. This includes standalone Nanobeads and MojoSort kits using Nanobeads. We have found that cells are functional without the need to detach the magnetic Nanobeads.
MojoSort Microbeads: These magnetic particles may be released following the detailed product-specific protocol.
- What is the size of your magnetic particles?
-
MojoSort Nanobeads: the average diameter is approximately 130 nm.
MojoSort Microbeads: the average diameter is approximately 1 μm.
- Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?
-
MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems. Please contact BioLegend Technical Service for more product-specific information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.
- What antibodies are present in the depletion cocktails provided for isolation kits?
-
Please contact our technical service team for further assistance.
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