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C57BL/6 mouse spleen was digested with collagenase and DNase I. A single cell suspension was prepared to isolate CD11c positive cells using MojoSort™ Mouse CD11c Nanobeads. Cells were stained with CD11c (clone N418) PE and MHC II (clone M5/114.15.2) APC. Dead cells were excluded by 7AAD. -
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| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 480077 | 20 tests | 232 CHF | ||||
| 480078 | 200 tests | 740 CHF | ||||
CD11c+ expressing cells are either selected or depleted by incubating your sample with the directly conjugated CD11c Nanobeads, after blocking with TruStain FcX (anti-mouse CD16/32 antibody). The magnetically labeled fraction is retained by the use of a magnetic separator. After collection or depletion of the CD11c+ cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.
MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.
Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage.
Kit Contents
- Kit Contents
-
For Cat# 480077:
- 1 vial containing 200 µl of Mouse CD11c Nanobeads
- 2 vials containing 100 µl of TruStain FcX (anti-mouse CD16/32 antibody)
For Cat# 480078:
- 2 vials of 1 ml each of Mouse CD11c Nanobeads
- 2 vials of 1 ml each of TruStain FcX (anti-mouse CD16/32 antibody)
Product Details
- Verified Reactivity
- Mouse
- Formulation
- Aqueous solution containing BSA and 0.05% sodium azide.
- Preparation
- Antibody conjugated particles: protein-coated magnetic nanobeads.
- Storage & Handling
- Mouse CD11c Nanobeads should be stored undiluted at 2°C and 8°C. TruStain FcX (Anti-mouse CD16/32 Antibody) should be stored undiluted at 2°C and 8°C.
- Application
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Cell Separation (MojoSort™) - Quality tested
- Recommended Usage
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10 µl Mouse CD11c Nanobeads for 1x107 cells in 100 µL of buffer.
10 µl of TruStain FcX (Anti-mouse CD16/32 antibody) for 1x107 cells in 100 µl of buffer.
- Application Notes
-
This kit is designed for the positive selection or depletion of mouse CD11c+ cells from lymphoid tissues.
Antibody or cocktail dilution to use in column: N/A
Nanobead dilution to use in columns: 50X
Antigen Details
- Biology Area
- Immunology, Innate Immunity, Costimulatory Molecules
- Molecular Family
- CD Molecules
- Gene ID
- NA
- UniProt
- View information about CD11c on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- Is there a way to detach your magnetic particles from the cell surface?
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MojoSort™ Nanobeads: These magnetic particles cannot be removed from cells. This includes standalone Nanobeads and MojoSort kits using Nanobeads. We have found that cells are functional without the need to detach the magnetic Nanobeads.
MojoSort Microbeads: These magnetic particles may be released following the detailed product-specific protocol.
- What is the size of your magnetic particles?
-
MojoSort Nanobeads: the average diameter is approximately 130 nm.
MojoSort Microbeads: the average diameter is approximately 1 μm.
- Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?
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MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems. Please contact BioLegend Technical Service for more product-specific information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.
- What antibodies are present in the depletion cocktails provided for isolation kits?
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Please contact our technical service team for further assistance.
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