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Mouse MDSC Flow Cocktail 1 with Isotype Ctrl

Pricing & Availability
Clone
M1/70; RB6-8C5; 1A8;
Regulatory Status
RUO
Other Names
Myeloid derived suppressor cells flow kit, CD11b/Ly6G/Gr-1
Isotype
Rat IgG2a/Rat IgG2b
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Product Citations
publications
MDSC_cocktail_Mouse_Antibodies_FC_1_120313
C57BL/6 mouse bone marrow cells were stained with mouse MDSC Flow Cocktail 1 (CD11b PE/Gr-1 APC/Ly-6G FITC).
  • MDSC_cocktail_Mouse_Antibodies_FC_1_120313
    C57BL/6 mouse bone marrow cells were stained with mouse MDSC Flow Cocktail 1 (CD11b PE/Gr-1 APC/Ly-6G FITC).
  • MDSC_cocktail_Mouse_Antibodies_FC_2_120313
Cat # Size Price Quantity Check Availability Save
147001 25 tests 347 CHF
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Description

This mouse MDSC Flow Cocktail 1 is composed of CD11b, Gr-1, and Ly-6G antibodies and corresponding isotype controls. MDSCs have been reported with CD11b and Gr-1 phenotype. MDSCs are a heterogenous population of cells that can  affect T cell function. Two different subsets of MDSC exist: CD11b+Ly-6G+Ly-6Clow (granulocytic MDSC) and CD11b+Ly-6G-Ly-6Chi (monocytic MDSC). These cells have been identified in a variety of pathological conditions including cancer and autoimmune disease.

CD11b: CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen.

Gr-1: Gr-1 is a 21-25 kD protein also known as Ly-6G/Ly-6C. This myeloid differentiation antigen is a glycosylphosphatidylinositol (GPI)-linked protein expressed on granulocytes and macrophages. In bone marrow, the expression levels of Gr-1 directly correlate with granulocyte differentiation and maturation; Gr-1 is also transiently expressed on bone marrow cells in the monocyte lineage. Immature Myeloid Gr-1+ cells play a role in the development of antitumor immunity.

Ly-6G: Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.
 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
This reagent is a multicolor cocktail of PE conjugated CD11b (clone M1/70), APC conjugated Gr-1 (clone RB6-8C5), and FITC conjugated Ly-6G (clone 1A8) at optimal concentration for three-color flow cytometric analysis.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of these antibodies is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Materials provided:

1. Mouse MDSC Flow Cocktail (containing CD11b PE/Gr-1 APC/Ly-6G FITC), 25 tests
2. Mouse MDSC Isotype Control Cocktail (containing rat IgG2b PE/rat IgG2b APC/rat IgG2a FITC), 25 tests

Materials not provided:

Cell Staining Buffer (Cat. No. 420201)
Single color compensation controls 

Product Citations
  1. Miyake M, et al. 2019. Cancer Sci. 110:3315. PubMed
RRID
AB_3097685 (BioLegend Cat. No. 147001)

Antigen Details

Biology Area
Immunology
Antigen References

1. Fleming TJ, et al. 1993. J. Immunol. 151:2399.
2. Daley JM, et al. 2008. J. Leukocyte Biol. 83:1.
3. Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205.
4. Barclay A, et al. 1997. The Leukocyte Antigen Factsbook Academic Press.
5. Springer TA. 1994. Cell 76:301.
6. Coxon A, et al. 1996. Immunity 5:653.
7. Brummer E, et al. 1984. J. Leukocyte Biol. 36:505.
8. Stoppacciaro A, et al. 1993. J. Exp. Med. 178:151.

Gene ID
1604 View all products for this Gene ID 17067 View all products for this Gene ID 54664 View all products for this Gene ID
Go To Top Version: 3    Revision Date: 06.01.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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