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Mouse Naïve/Memory T cell ID Panel

Pricing & Availability
Clone
17A2; RM4-5; MEL-14; IM7;
Regulatory Status
RUO
Other Names
CD3, CD4, CD44, CD62L, TCM, TEM
Isotype
Rat IgG2a/Rat IgG2b
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Product Citations
publications
Mouse_Naive_Memory_T_Cell_1_091724
C57BL/6 splenocytes were stained using the Mouse Naïve/Memory T cell ID Panel consisting of CD3 APC/Cyanine7, CD4 PerCP/Cyanine5.5, CD62L APC and CD44 PE. Left dot plot depicts CD3+CD4+ T cells. Right plot shows CD44loCD62Lhi naïve cells and CD44hiCD62Llo memory T cells (in gated CD3+CD4+ cell population).
  • Mouse_Naive_Memory_T_Cell_1_091724
    C57BL/6 splenocytes were stained using the Mouse Naïve/Memory T cell ID Panel consisting of CD3 APC/Cyanine7, CD4 PerCP/Cyanine5.5, CD62L APC and CD44 PE. Left dot plot depicts CD3+CD4+ T cells. Right plot shows CD44loCD62Lhi naïve cells and CD44hiCD62Llo memory T cells (in gated CD3+CD4+ cell population).
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147501 50 tests 639 CHF
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Description

In mice, CD4+ T cells can be divided into naïve and memory cells based on expression of adhesion molecules CD44 and CD62L. Naïve T cells exhibit high levels of CD62L and low expression of CD44, whereas memory T cells are identified by high CD44 and low CD62L expression. This panel includes antibodies against CD3, CD4, CD62L and CD44, which can be used together to identify naïve and memory T cell subsets in mice. Alterations in these subsets are evident in older mice following antigenic stimulation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
This reagent contains one vial each of APC/Cyanine7 conjugated anti-CD3 (clone 17A2), PerCP/Cyanine5.5 conjugated anti-CD4 (clone RM4-5), APC conjugated anti-CD62L (clone MEL-14), and PE conjugated anti-CD44 (clone IM7) at optimal concentration for four-color flow cytometric analysis.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of these antibodies is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product consists of four antibody vials: CD3 APC/Cyanine7, CD4 PerCP/Cyanine5.5, CD62L APC, and CD44 PE.

Product Citations
  1. Balakrishnan PB, et al. 2022. Nano Res. 15:2300. PubMed
  2. Cai B, et al. 2021. Mol Cancer. 20:165. PubMed
RRID
AB_3097690 (BioLegend Cat. No. 147501)

Antigen Details

Cell Type
Mesenchymal Stem Cells
Biology Area
Immunology, Innate Immunity, Stem Cells
Molecular Family
Adhesion Molecules
Antigen References
  1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
  2. Haynes BF, et al. 1991. Cancer Cells 3:347. 
  3. Goldstein LA, et al. 1989. Cell 56:1063. 
  4. Kishimoto TK, et al. 1990. Proc. Natl. Acad. Sci. USA 87:2244. 
  5. Tedder TF, et al. 1995. J. Exp. Med. 181:2259. 
  6. Davis MM. 1990. Annu. Rev. Biochem. 59:475. 
  7. Weiss A, et al. 1994. Cell 76:263. 
  8. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579. 
  9. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.
Gene ID
12502 View all products for this Gene ID 12504 View all products for this Gene ID 20343 View all products for this Gene ID 12505 View all products for this Gene ID
Go To Top Version: 2    Revision Date: 09.17.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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