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Neuron Marker Antibody Sampler Kit

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RUO
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Please refer to individual product datasheets.
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publications
1-SMI-32_A594_NeuroH_Antibody_11_122817
IHC staining of Alexa Fluor® 594 anti-Neurofilament H (NF-H), Nonphosphorylated antibody (clone SMI 32) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3 (Cat. No. 927801), the tissue was blocked with a buffer containing 5% Normal Goat Serum, 1% BSA and 0.25% Triton-X-100 in PBS. The tissue was then incubated with 5 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 40x objective. Scale bar: 50 µm
  • 1-SMI-32_A594_NeuroH_Antibody_11_122817
    IHC staining of Alexa Fluor® 594 anti-Neurofilament H (NF-H), Nonphosphorylated antibody (clone SMI 32) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3 (Cat. No. 927801), the tissue was blocked with a buffer containing 5% Normal Goat Serum, 1% BSA and 0.25% Triton-X-100 in PBS. The tissue was then incubated with 5 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 40x objective. Scale bar: 50 µm
  • 2-SMI-32_A594_NeuroH_Antibody_33_122817
    ICC staining of Alexa Fluor® 594 anti-Neurofilament H (NF-H), Nonphosphorylated antibody (clone SMI 32) on SH-SY5Y neuroblastoma cells. The cells were fixed with 100% Methanol, permeabilized with 0.1% Triton-X-100 and 0.1% BSA in PBS, and blocked with 2% Normal Goat Serum and 0.02% BSA in PBS. The cells were then incubated with 5 µg/mL of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 40x objective. Scale bar: 50 µm
  • 3-SMI-52_A488_MAP2_Antibody_IHC-P_1_122017
    IHC staining of Alexa Fluor® 488 anti-MAP2 antibody (clone SMI 52) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 10X objective. Scale bar: 100 µm
  • 4-NSE-P1_A647_NSE_Antibody_1_IHC-P_122117
    IHC staining of Alexa Fluor® 647 anti-NSE antibody (clone NSE-P1) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. The image was captured with a 40x objective. Scale bar: 20 µm.
  • 5-1B7_PURE_FOX3_NeuN_Antibody_IHC_3_010418
    IHC staining of purified anti-FOX3 (NeuN) antibody (clone 1B7) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 10X objective. Scale bar: 100 µm
  • 6-TUJ1_A594_Tubulin-beta_IF_092517
    ICC staining of Alexa Fluor® 594 anti-Tubulin β 3 (TUBB3) (clone TUJ1) on SH-SY5Y neuroblastoma cells. The cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100, and blocked with 2% normal goat serum. The cells were then stained with 1 µg/ml of the primary antibody for three hours at room temperature. Nuclei were counterstained with Hoechst 33342. The image was captured with a 60X objective.
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899905 1 kit 516 CHF
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Description

The Neuron Marker Antibody Sampler Kit offers flexibility for sampling and detection of specific cellular structures or compartments of neurons. These antibodies are highly specific for neuronal targets, and provide an easy and rapid solution for multiplexing in ICC or IHC applications.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
Specificity Format Clone Size Reactivity Isotype
Anti-Neurofilament H (NF-H), Nonphosphorylated Alexa Fluor® 594 SMI 32 25 μg Human, Mouse, Rat, Other mammalian Mouse IgG1, κ
Anti-MAP2 Alexa Fluor® 488 SMI 52 25 μg Mouse, Rat Mouse IgG1, κ
 Anti-NSE Alexa Fluor® 647 NSE-P1 25 μg Human Mouse IgG1, κ
 Anti-FOX3 (NeuN) Purified 1B7 25 μl Human, Cow, Pig, Mouse, Rat, and other mammals Mouse IgG2a
 Anti-Tubulin β 3 (TUBB3) Alexa Fluor® 594 TUJ1 25 μg Human, Mouse, Rat Mouse IgG2a, κ

* For detailed information about each specificity, please refer to the datasheets of the individual products. 

Product Details

Formulation
Please refer to individual product datasheets.
Preparation
Please refer to individual product datasheets.
Storage & Handling
Upon receipt, store undiluted at 2-8°C.
Application

IHC-P, ICC - Quality tested
Recommended Usage

Each lot of antibodies in this kit is quality control tested by immunohistochemical staining on formalin-fixed paraffin-embedded tissue or immunocytochemistry. For immunohistochemistry, the suggested uses of these reagents are as follows:

Anti-Neurofilament H (NF-H), Nonphosphorylated: 5.0 - 10 µg/ml
Anti-MAP2: 1.0 - 5.0 µg/ml
Anti-NSE: 1.0 - 5.0 µg/ml
Anti-FOX3 (NeuN): 0.1 - 0.5 µg/ml

For immunocytochemistry, the suggested uses of these reagents are as follows:

Anti-Tubulin β 3 (TUBB3): 0.5 - 1.0 µg/ml

It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For verified or reported applications for these antibodies, please see individual product datasheets.

Related FAQs

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Go To Top Version: 2    Revision Date: 06.18.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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