Pacific Blue™ anti-human CD38 Antibody, CD38, HIT2

Pricing & Availability

Clone: HIT2 (See other available formats)
Regulatory Status: RUO
Workshop: III 155
Other Names: T10, ADP-ribosyl cyclase
Isotype: Mouse IgG1, κ
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Product Citations: publications

HIT2_Pacific-Blue_CD38_Antibody_092622 — Human peripheral blood lymphocytes were stained with anti-human CD19 PE and anti-human CD38 (clone HIT2) Pacific Blue™ (left) or mouse IgG1, κ Pacific Blue™ isotype control (right).

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303555

25 tests

105 CHF

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303556

100 tests

265 CHF

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Description

CD38 is a 45 kD type II transmembrane glycoprotein also known as T10. It is an ADP-ribosyl hydrolase expressed at variable levels on hematopoietic cells and in some non-hematopoietic tissues (such as brain, muscles, and kidney). In humans, it is expressed at high levels on plasma cells and activated T and B cells. By functioning as both a cyclase and a hydrolase, CD38 mediates lymphocyte activation, adhesion, and the metabolism of cADPR and NAADP. CD31 is the ligand of CD38.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Reported Reactivity: Chimpanzee, Horse, Cow
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections⁶ and spatial biology (IBEX)^10,11.
RRID: AB_2927895 (BioLegend Cat. No. 303555)
AB_2927895 (BioLegend Cat. No. 303556)

Antigen Details

Structure: ADP-ribosyl cyclase, ectoenzyme, type II glycoprotein, 45 kD
Distribution: T cells, B cells, NK, myeloid, plasma, and dendritic cells
Function: Ecto-ADP-ribosyl cyclase, calcium signaling, cell activation
Ligand/Receptor: CD31, hyaluronic acid
Cell Type: B cells, Dendritic cells, NK cells, Plasma cells, T cells
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Ferrero E, et al. 1999. J. Leukoc. Biol. 65:151.
2. Lund F, et al. 1995. Immunol. Today 16:469.
Gene ID: 952 View all products for this Gene ID
UniProt: View information about CD38 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Pathways

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Other Formats

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Description	Clone	Applications
APC anti-human CD38	HIT2	FC
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PE/Cyanine7 anti-human CD38	HIT2	FC
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PerCP anti-human CD38	HIT2	FC
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Alexa Fluor® 700 anti-human CD38	HIT2	FC,SB
Brilliant Violet 421™ anti-human CD38	HIT2	FC
Brilliant Violet 711™ anti-human CD38	HIT2	FC
Brilliant Violet 785™ anti-human CD38	HIT2	FC
Brilliant Violet 605™ anti-human CD38	HIT2	FC
APC/Cyanine7 anti-human CD38	HIT2	FC
Purified anti-human CD38 (Maxpar® Ready)	HIT2	FC,CyTOF®
PE/Dazzle™ 594 anti-human CD38	HIT2	FC
PE anti-human CD38	HIT2	FC
Brilliant Violet 510™ anti-human CD38	HIT2	FC
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TotalSeq™-A0389 anti-human CD38	HIT2	PG
TotalSeq™-C0389 anti-human CD38	HIT2	PG
APC/Fire™ 750 anti-human CD38	HIT2	FC
TotalSeq™-B0389 anti-human CD38	HIT2	PG
APC/Fire™ 810 anti-human CD38	HIT2	FC
Spark NIR™ 685 anti-human CD38 Antibody	HIT2	FC
TotalSeq™-D0389 anti-human CD38	HIT2	PG
PerCP/Cyanine5.5 anti-human CD38	HIT2	FC
APC anti-human CD38	HIT2	FC
APC/Fire™ 750 anti-human CD38	HIT2	FC
PE/Cyanine7 anti-human CD38	HIT2	FC
GMP PE anti-human CD38	HIT2	FC
GMP FITC anti-human CD38	HIT2	FC
Pacific Blue™ anti-human CD38	HIT2	FC
Pacific Blue™ anti-human CD38	HIT2	FC
GMP APC/Fire™ 750 anti-human CD38	HIT2	FC
GMP PE/Dazzle™ 594 anti-human CD38	HIT2	FC
GMP APC anti-human CD38	HIT2	FC
GMP PE/Cyanine7 anti-human CD38	HIT2	FC
Spark Blue™ 515 anti-human CD38	HIT2	FC
GMP Pacific Blue™ anti-human CD38	HIT2	FC
Spark Violet™ 423 anti-human CD38	HIT2	FC
Brilliant Violet 650™ anti-human CD38	HIT2	FC
Spark PLUS B550™ anti-human CD38	HIT2	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 09.26.2022

For Research Use Only. Not for diagnostic or therapeutic use.

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