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Pacific Blue™ anti-human CD89 Antibody

Pricing & Availability
Clone
A59 (See other available formats)
Regulatory Status
RUO
Workshop
V MR30
Other Names
FcαR
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A59_PB_CD89_Antibody_071718
Human peripheral blood granulocytes were stained with anti-human CD89 Pacific Blue™ (clone A59, filled histogram) or mouse IgG1, κ Pacific Blue™ isotype control (open histogram).
  • A59_PB_CD89_Antibody_071718
    Human peripheral blood granulocytes were stained with anti-human CD89 Pacific Blue™ (clone A59, filled histogram) or mouse IgG1, κ Pacific Blue™ isotype control (open histogram).
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Cat # Size Price Quantity Check Availability Save
354119 25 tests 153 CHF
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354120 100 tests 364 CHF
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Description

CD89, also known as FcαR, is a 55-100 kD glycosylated protein.  It belongs to the immunoglobulin gene family.  It is expressed on granulocytes, monocytes, and macrophages but is absent on T cells.  It can interact with IgA aggregates and plays an important role in IgA mediated immune responses.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Ag8.653 myeloma cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Monteiro RC, et al. 1992. J. Immunol. 148:1764.
  2. Shen L. 1992. J. Leukoc. Biol. 51:373.
  3. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  4. Rogers KA, et al. 2004. Immunology 113:178.
RRID
AB_2750068 (BioLegend Cat. No. 354119)
AB_2750068 (BioLegend Cat. No. 354120)

Antigen Details

Structure
Member of the immunoglobulin gene family and is a transmembrane glycoprotein, 55-100 kD
Distribution

Granulocytes, neutrophils, eosinophils, monocytes, macrophages

Function
Interacts with IgA opsonized targets and induces immunological processes such as phagocytosis, ADCC, and release of inflammatory mediators
Ligand/Receptor
IgA (monomeric, dimeric, polymeric and secretory IgA)
Cell Type
Eosinophils, Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area
Immunology
Molecular Family
CD Molecules, Fc Receptors
Antigen References

1. Patry C, et al. 1996. J. Immunol. 156:4442.
2. de Wit, et al. 1995. J. Immunol. 155:1203.
3. Honorio-França AC, et al. 2001. J. Leukoc. Biol. 69:289.

Gene ID
2204 View all products for this Gene ID
UniProt
View information about CD89 on UniProt.org
Go To Top Version: 1    Revision Date: 07.17.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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