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Pacific Blue™ anti-mouse CD105 Antibody

Pricing & Availability
Clone
MJ7/18 (See other available formats)
Regulatory Status
RUO
Other Names
Endoglin
Isotype
Rat IgG2a, κ
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Product Citations
publications
MJ7slash18_PB_092109
bEND.3 cells stained with MJ7/18 Pacific Blue™
  • MJ7slash18_PB_092109
    bEND.3 cells stained with MJ7/18 Pacific Blue™
Compare all formats See Pacific Blue™ spectral data
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120411 25 µg 101 CHF
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120412 100 µg 229 CHF
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Description

CD105 is a 90 kD homodimeric type I integral membrane glycoprotein, also known as endoglin. It is expressed on endothelial cells (especially on angiogenic endothelial cells) and upregulated by hypoxia, activated monocytes, macrophages, bone marrow stromal cells, and some cytotrophoblasts. CD105 is a receptor for TGF-β1, TGF-β3 and modulates TGF-β signaling by interacting with TGF-β receptors I and/or II. CD105 also binds other growth factors such as actvin A, BMP-2, and BMP-7. CD105 has been show to be a useful marker for identifying proliferating endothelium involved in tumor angiogenesis and can be used for tumor imaging and prognosis, and has therapeutic potential for some solid tumors and other angiogenic diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Inflamed mouse skin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications include: immunoprecipitation, Western blotting, and immunofluorescence histochemistry or immunohistochemistry of acetone-fixed frozen sections2-4.

Application References

(PubMed link indicates BioLegend citation)
  1. Ge AZ and Butcher EC. 1994. Gene 138:201.
  2. Baluk P, et al. 2003. Am. J. Pathol. 163:1801. (IHC)
  3. Takahashi T, et al. 2003. Mol. Cell Biol. 23:1817. (IHC)
  4. Savinov AY, et al. 2003. J. Exp. Med. 197:643. (IHC)
Product Citations
  1. Lang J, et al. 2020. Nat Commun. 11:1338. PubMed
  2. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
  3. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  4. Wu Z, et al. 2023. EMBO Rep. 24:e56524. PubMed
  5. Mulcrone PL, et al. 2023. Sci Rep. 13:8436. PubMed
  6. Oh D, et al. 2012. J Immunol. 188:2207. PubMed
  7. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  8. Marinescu CI, et al. 2021. Stem Cell Res Ther. 12:319. PubMed
  9. van Gastel N, et al. 2020. Cell Metabolism. 32(3):391-403.e6. PubMed
  10. Fischer KC, et al. 2021. Cell Death Dis. 12:28. PubMed
  11. Tournaire G, et al. 2020. Bone. 115259:133. PubMed
  12. Spevak CC, et al. 2020. Cell Stem Cell. 26(5):755-765. PubMed
RRID
AB_1877185 (BioLegend Cat. No. 120411)
AB_1877185 (BioLegend Cat. No. 120412)

Antigen Details

Structure
Type I integral membrane protein, homodimer, TGF-β type III receptor family member
Distribution

Endothelial cells, activated monocytes, macrophages, stromal cells, some cytotrophoblast

Function
Suppresses TGF-β signaling, angiogenesis
Ligand/Receptor
TGF-β1, TGF-β3
Cell Type
Endothelial cells, Macrophages, Mesenchymal Stem Cells, Monocytes
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Gougos A and M. Letarte 1988. J. Immunol. 141:1925.
2. Cheifetz S, et al. 1992. J. Bio. Chem. 267:19027.
3. Barbara NP, et al. 1999. J. Bio. Chem. 274:584.
4. Lastres P, et al. 1992. Eur. J. Immunol. 22:393.
5. Duff S, et al. 2003. FASEB J. 17:984.
6. Warrington K, et al. 2005. Anticancer Res. 25:185.

Gene ID
13805 View all products for this Gene ID
UniProt
View information about CD105 on UniProt.org
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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