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Pacific Blue™ anti-mouse CD8b.2 Antibody

Pricing & Availability
Clone
53-5.8 (See other available formats)
Regulatory Status
RUO
Other Names
Lyt-3.2, Ly-3.2
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
53-5.8_PB_081210
C57BL/6 mouse splenocytes stained with 53-5.8 Pacific Blue™
  • 53-5.8_PB_081210
    C57BL/6 mouse splenocytes stained with 53-5.8 Pacific Blue™
Compare all formats See Pacific Blue™ spectral data
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140414 100 µg 288 CHF
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Description

CD8b is the 32 kD β chain of CD8, also known as Lyt-3.2 or Ly-3.2. It is a member of the Ig superfamily expressed as a heterodimer with the CD8α chain on a subset of MHC class I-restricted T cells and most thymocytes. CD8 is a co-receptor for the TCR complex involved in T cell activation. The antibody 53-5.8 is specific for Ly-3.2 and has low reactivity with Ly-3.1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence3, immunohistochemical staining4 of frozen tissue section using dry ice-isopentane method and immunoprecipitation5.

Application References

(PubMed link indicates BioLegend citation)
  1. Ledbetter J, et al. 1979. Immunol. Rev. 47:63.
  2. Ledbetter J, et al. 1980. J. Exp. Med. 152:280.
  3. Vremec D, et al. 2000. J. Immunol. 164:2978. (IF)
  4. Lawrence D, et al. 1999. J. Virol. 73:1795. (IHC)
  5. Bosselut R, et al. 1999. J. Exp. Med. 190:1517. (IP)
Product Citations
  1. Kosack L, et al. 2017. Sci Rep. 7:11289. PubMed
  2. Lercher A, et al. 2019. Immunity. 51:1074. PubMed
  3. Nika L, et al. 2019. Vaccines (Basel). 7. PubMed
  4. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  5. Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed
RRID
AB_10641278 (BioLegend Cat. No. 140414)

Antigen Details

Structure
Ig superfamily, CD8β chain, 32 kD
Distribution

Majority of thymocytes, cytotoxic T cell subsets

Function
Co-receptor of TCR
Ligand/Receptor
MHC class I
Cell Type
T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules, TCRs
Antigen References

1. Ledbetter J, et al. 1981. J. Exp. Med. 153:1503.
2. Renard V, et al. 1996. J. Exp. Med. 184:2439.

Gene ID
12526 View all products for this Gene ID
UniProt
View information about CD8beta.2 on UniProt.org
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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