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Pacific Blue™ anti-mouse Ly-49A Antibody

Pricing & Availability
Clone
YE1/48.10.6 (See other available formats)
Regulatory Status
RUO
Other Names
Klra1, A1, killer cell lectin-like receptor, subfamily A, member 1
Isotype
Rat IgG2a, κ
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Product Citations
publications
YE1slash48_PacBlue_081506
C57BL/6 mouse splenocytes stained with anti-Ly49G FITC and YE1/48.10.6 Pacific Blue™
  • YE1slash48_PacBlue_081506
    C57BL/6 mouse splenocytes stained with anti-Ly49G FITC and YE1/48.10.6 Pacific Blue™
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116810 100 µg 229 CHF
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Description

Ly-49A is a 44 kD member of the C-type lectin/Ly-49 family proteins. It is a type-II transmembrane, disulfide-linked homodimer with C-type lectin extracellular domains. Ly-49A is present on a subset of NK and NK-T cells. Ly-49A, like other members of this family, inhibits NK cell activation through the recognition and interaction of certain MHC class I haplotypes on potential target cells. The Ly-49A receptor recognizes H-2Dd and H-2Dk, triggering phosphorylation of the ITIM motif in the cytoplasmic domain and recruitment of SHP-1 and SHP-2. The YE1/48.10.6 antibody reacts with NK cells from all mouse strains tested and reverses the inhibition of NK cytotoxicity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Con A activated splenic T cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2

.

Application References

(PubMed link indicates BioLegend citation)
  1. Takei F. 1983. J. Immunol. 130:2794.
  2. Chan P, et al. 1989. J. Immunol. 142:1727.
Product Citations
  1. Adams NM et al. 2018. Immunity. 48(6):1172-1182 . PubMed
  2. Wiedemann GM, et al. 2020. Cell Rep. 33:108498. PubMed
  3. Yang E, et al. 2016. J Immunol. 197: 934 - 941. PubMed
  4. Wagner AK, et al. 2022. iScience. 25:105137. PubMed
  5. Geary CD et al. 2018. Cell reports. 24(8):1949-1957 . PubMed
RRID
AB_572013 (BioLegend Cat. No. 116810)

Antigen Details

Structure
C-type lectin, Ly-49, ITIM, 44 kD
Distribution

NK and NK-T cells

Function
Inhibitory NK cell receptor
Ligand/Receptor
MHC Class I Molecules
Cell Type
NK cells, NKT cells
Biology Area
Immunology
Antigen References

1. Coles MC, et al. 2000. Eur. J. Immunol. 30:236.
2. Takei FJ, et al. 1997. Immunol. Rev. 155:67.
3. Hanke T, et al. 1999. Immunity 11:67.

Gene ID
16627 View all products for this Gene ID
UniProt
View information about Ly-49A on UniProt.org
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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