PE anti-Acetylated Lysine Antibody anti-Acetylated Lysine

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Clone: 15G10 (See other available formats)
Regulatory Status: RUO
Other Names: Proteins are acetylated by the reversible transfer of acetyl-CoA to the ε-amino group of lysine
Isotype: Mouse IgG2b, κ
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Product Citations: publications

15G10_PE_acetylated_lysine_Antibody_FC_1_013114 — Human cervical carcinoma cell line, HeLa, was incubated with (top) or without (bottom) Trichostatin A (100 ng/ml, overnight), then treated with FOXP3 Fix/Perm Buffer Set. Cells were then stained with anti-acetylated lysine (clone 15G10) PE (filled histogram) or mouse IgG2b, κ PE isotype control (open histogram).

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623404

100 tests

335 CHF

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Description

Proteins are reversibly and dynamically acetylated on the ε-amino group of lysine by acetyltransferases and deacetylated by deacetylases. This post-translational modification can regulate protein function (interactions with other proteins and DNA binding). Histones and transcription factors (PCAF, p53, p300, etc) appear to be the major targets of acetyltransferases. Acetylation is usually associated with chromatin remodeling and transcriptional activation, although in some cases (telomeres) it is associated with gene silencing. The 15G10 antibody recognizes acetylated lysine residues on proteins.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Species independent
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Acetylated protein mixture
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_2563126 (BioLegend Cat. No. 623404)

Antigen Details

Structure: Proteins are acetylated by the reversible transfer of acetyl-CoA to the ε-amino group of lysine
Function: Post-translational modification of proteins by acetyltransferases and deacetylated by deacetylases to regulate protein-protein and protein-DNA interactions
Biology Area: Cell Biology, Chromatin Remodeling/Epigenetics
Antigen References: 1. Wang S, et al. 2004. Novartis Found. Symp. 259:238.
2. Legube G, et al. 2003. EMBO Rep. 4:944.
3. Chen LF, et al. 2003. J. Mol. Med. 81:549.
Gene ID: 8850 View all products for this Gene ID
UniProt: View information about Acetylated Lysine on UniProt.org

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Related FAQs

What type of PE do you use in your conjugates?: We use R-PE in our conjugates.

Other Formats

View All Acetylated Lysine Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-Acetylated Lysine	15G10	WB,ICC
PE anti-Acetylated Lysine	15G10	ICFC
Alexa Fluor® 647 anti-Acetylated Lysine	15G10	ICFC
PE/Cyanine7 anti-Acetylated Lysine	15G10	ICFC

Certificate of Analysis

Go To Top Version: 2 Revision Date: 09.19.2023

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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