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PE anti-AKT Phospho (Ser473) Antibody

Pricing & Availability
Clone
A21001C (See other available formats)
Regulatory Status
RUO
Other Names
Protein kinase B alpha, PRK-BA, PKB, Serine/Threonine specific kinase RAC alpha, Protein kinase Akt, RAC
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A21001C_PE_AKT_072823_Antibody_072823
Jurkat cells untreated (positive control, filled histogram), or treated with 1 µM wortmannin for 2 hours (negative control, open histogram) were fixed and permeabilized using the True-Phos™ Perm Buffer Set (Cat. No. 425401) and intracellularly stained with PE anti-AKT Phospho (Ser473) (clone A21001C) or PE mouse IgG1, κ isotype control (open histogram, dashed line) (representative histogram for untreated or treated cells) (Cat. No. 400113).
  • A21001C_PE_AKT_072823_Antibody_072823
    Jurkat cells untreated (positive control, filled histogram), or treated with 1 µM wortmannin for 2 hours (negative control, open histogram) were fixed and permeabilized using the True-Phos™ Perm Buffer Set (Cat. No. 425401) and intracellularly stained with PE anti-AKT Phospho (Ser473) (clone A21001C) or PE mouse IgG1, κ isotype control (open histogram, dashed line) (representative histogram for untreated or treated cells) (Cat. No. 400113).
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606553 25 tests 130 CHF
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606554 100 tests 380 CHF
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Description

AKT (also known as protein kinase B alpha) is a 60 kD serine/threonine-specific kinase containing a pleckstrin domain. AKT plays a critical role in controlling survival and apoptosis. This kinase is ubiquitously expressed and translocates to the membrane upon cell activation. It is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI 3-kinase. AKT is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2, responsible for phosphorylation of AKT at Ser473, has been identified as a mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1. Activated AKT phosphorylates a wide range of substrates including transcription factors (e.g. FOXO1), kinases (GSK-3beta, Raf-1, ASK, Chk1) and other proteins with important signaling roles (e.g. Bad, MDM2). AKT promotes cell survival by inhibiting apoptosis through phosphorylation of several targets, including Bad, forkhead transcription factor, c-Raf and caspase-9. AKT also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor. More importantly, AKT phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide of human AKT phosphorylated at Ser473
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

This clone was tested by Western Blot using lysates prepared from HeLa cells untreated or treated with IGF-1, and NIH/3T3 cell untreated or treated with PDGF. 

This clone is suitable for ICC in cells treated with PFA and permeabilized with either methanol or Triton X-100.

RRID
AB_3068251 (BioLegend Cat. No. 606553)
AB_3068251 (BioLegend Cat. No. 606554)

Antigen Details

Structure
AKT is a 480 amino acid protein with a predicted molecular weight of 56 kD
Distribution

Ubiquitously expressed, translocates to the membrane upon activation

Function
Catalytically inactive multimeric complex. AKT is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473 by PDK2.
Interaction
Interacts through pleckstrin homology domain with second messengers. Interacts with AKTIP, CDKN1B.
Antigen References
  1. Jacinto E, et al. 2006. Cell. 127:125-37.
  2. Inoki K, et al. 2002. Nat Cell Biol. 4:648-57.
  3. Franke TF, et al. 1995. Cell. 81:727-36.
  4. Brunet A, et al. 1999. Cell. 96:857-68.
  5. Yang WL, et al. 2010. Cell Cycle. 9:487-97.
Gene ID
207 View all products for this Gene ID
UniProt
View information about AKT Phospho Ser473 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 07.28.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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