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PE anti-EGFR Phospho (Tyr845) Recombinant Antibody

Pricing & Availability
Clone
QA19A93 (See other available formats)
Regulatory Status
RUO
Other Names
Epidermal Growth Factor Receptor, Receptor Tyrosine-Protein Kinase ErbB-1, Erb-B2 Receptor Tyrosine Kinase 1, ERBB1, NISBD2, PIG61, MENA
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
QA19A93_PE_EGFR-Phospho_Antibody_031023
Human A-431 cells, 24 hours serum starved and were either untreated (open histogram) or treated with 300 ng/mL recombinant human EGF (Cat. No. 585506) for 5 minutes at 37°C (filled histogram), were fixed using Fixation Buffer (Cat. No. 420801), then permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401). Cells were then intracellularly stained with anti-EGFR Phospho (Tyr845) recombinant (clone QA19A93) PE.
  • QA19A93_PE_EGFR-Phospho_Antibody_031023
    Human A-431 cells, 24 hours serum starved and were either untreated (open histogram) or treated with 300 ng/mL recombinant human EGF (Cat. No. 585506) for 5 minutes at 37°C (filled histogram), were fixed using Fixation Buffer (Cat. No. 420801), then permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401). Cells were then intracellularly stained with anti-EGFR Phospho (Tyr845) recombinant (clone QA19A93) PE.
See PE spectral data
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943403 25 tests 171 CHF
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943404 100 tests 288 CHF
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Description

Epidermal growth factor receptor (EGFR) is a receptor tyrosine kinase that links extracellular mitogenic ligand binding to complex downstream signaling cascades. Initial ligand binding results in receptor oligomerization and autophosphorylation of multiple tyrosine residues within cytosolic domains of the protein. Phosphorylation at tyrosine 845, which is found in the kinase activation loop, stabilizes the active conformation and leads to the recruitment of adaptor proteins and other downstream effectors. EGFR stimulation leads to activation of RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCγ-PKC and STAT signaling cascades, and constitutive activation of the receptor promotes tumorigenesis in multiple cancers.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Synthetic peptide corresponding to human EGFR phosphorylated at tyrosine 845
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

This clone was tested for ICC on A-431 cells treated with or without EGF. We failed to detect any EGFR Phospho (Tyr845) staining using the following fixation and permeabilization methods:

  • 4% PFA plus Triton X-100
  • 4% PFA plus methanol
  • Methanol only
This clone failed to immunoprecipitate EGFR Phospho (Tyr845) from whole cell extracts prepared from EGF-stimulated A-431 cells.

This recombinant antibody clone is derived from clone A18028A.

This clone is predicted to recognize the region corresponding to EGFR Phospho (Tyr845) in both rat (Tyr846) and mouse (Tyr847) orthologs due to complete sequence conservation with the immunizing peptide.
RRID
AB_2936764 (BioLegend Cat. No. 943403)
AB_2936764 (BioLegend Cat. No. 943404)

Antigen Details

Structure
EGFR is a 1,210 amino acid protein with a predicted molecular weight of 134 kD; the processed form is 1,186 amino acids in length.
Distribution

Plasma membrane/Ubiquitously expressed

Function
EGFR signaling, cell signaling
Ligand/Receptor
EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF
Cell Type
Epithelial cells
Biology Area
Cell Biology, Cell Proliferation and Viability, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase
Antigen References
1. Hunter T and Cooper JA. 1981. Cell. 24:741.
2. Gill GN and Lazar CS. 1981. Nature. 293:305.
3. Reynolds FH, et al. 1981. Nature. 292:259
4. Biscardi, et al. 1999. J. Biol. Chem. 274:8335.
5. Zhou M, et al. 2013. Cancer Res. 23:7056-67
Gene ID
1956 View all products for this Gene ID
UniProt
View information about EGFR on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 03.10.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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