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PE anti-GATA1 Antibody

Pricing & Availability
Clone
P84F5 (See other available formats)
Regulatory Status
RUO
Other Names
GATA Binding Protein 1, Erythroid Transcription Factor, Globin Transcription Factor 1, NFE1, XLTT
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
P84F5_PE_GATA1_ICFC_052924
HEL cells (positive control, filled histogram) and HeLa cells (negative control, open histogram) were fixed and permeabilized using True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) and intracellularly stained with PE anti-GATA1 (clone P84F5) or PE mouse IgG2b, κ isotype control (open histogram, dashed line) (representative histogram for both cell lines) (Cat. No. 400312).
  • P84F5_PE_GATA1_ICFC_052924
    HEL cells (positive control, filled histogram) and HeLa cells (negative control, open histogram) were fixed and permeabilized using True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) and intracellularly stained with PE anti-GATA1 (clone P84F5) or PE mouse IgG2b, κ isotype control (open histogram, dashed line) (representative histogram for both cell lines) (Cat. No. 400312).
Compare all formats See PE spectral data
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939103 25 tests 140 CHF
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939104 100 tests 355 CHF
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Description

The GATA family of transcription factors is comprised of six members characterized by their binding to the consensus DNA sequence (T/A)GATA(A/G). GATA1, which was the initially described member of the GATA family, is predominantly expressed in cells of the myeloid lineage such as erythrocytes, eosinophils, mast cells, and megacaryocytes. Over the course of erythroid differentiation, GATA2 activates expression of the functionally distinct GATA1; promoter-occupied GATA2 is replaced/exchanged at certain promoters with the GATA1 transcription factor, a process known as the GATA switch. Genes induced by GATA1 include HBB, HBG1/2, ALAS2 and HMBS. Haploinsufficient heterozygous GATA1 knockout mice spontaneously develop erythroblastic leukemia, and GATA1-null mice die early in development due to profound anemia.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full-length recombinant human GATA1 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

When using this clone for ICFC, we recommend using the True-Nuclear™ Transcription Factor Buffer Set.

RRID
AB_3106234 (BioLegend Cat. No. 939103)
AB_3106234 (BioLegend Cat. No. 939104)

Antigen Details

Structure
GATA1 is a 413 amino acid protein with a predicted molecular weight of 43 kD. Additional isoforms of 335 (35 kD) and 330 (34 kD) amino acids have been described.
Distribution

Erythroid and megakaryocytic cells/Nucleus

Function
Transcription Factor/Erythroid cell development and differentiation
Cell Type
Erythrocytes, Megakaryocytes
Biology Area
Cell Biology, Stem Cells, Transcription Factors
Antigen References
  1. Shimizu R, et al. 2008. Nat Rev Cancer. 8:279-87.
  2. Bresnick EH, et al. 2010. J Biol Chem. 285:31087-93.
  3. Katsumura, et al. 2017. Blood. 129:2092-2102.
Gene ID
2623 View all products for this Gene ID
UniProt
View information about GATA1 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 05.29.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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