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PE anti-His Tag Antibody

Pricing & Availability
Clone
J095G46 (See other available formats)
Regulatory Status
RUO
Other Names
Hexa histidine-tag, 6xHis-tag, 6XHis, 6His
Isotype
Mouse IgG2a, κ
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Product Citations
publications
J095G46_PE_His-Tag_Antibody_FC_1_061614.jpg
His-Tag transfected HEK293 cells were treated with Fixation Buffer (Cat. No. 420801) and Permeabilization Wash Buffer (Cat. No. 421002), and then stained with Anti-His-Tag (clone J095G46) PE (filled histogram) or mouse IgG2a, κ PE isotype control (open histogram).
  • J095G46_PE_His-Tag_Antibody_FC_1_061614.jpg
    His-Tag transfected HEK293 cells were treated with Fixation Buffer (Cat. No. 420801) and Permeabilization Wash Buffer (Cat. No. 421002), and then stained with Anti-His-Tag (clone J095G46) PE (filled histogram) or mouse IgG2a, κ PE isotype control (open histogram).
  • J095G46_PE_His-Tag_Antibody_FC_2_061614.jpg
    His-Tag non-transfected HEK293 cells were treated with Fixation Buffer (Cat. No. 420801) and Permeabilization Wash Buffer (Cat. No. 421002), and then stained with Anti-His-Tag (clone J095G46) PE (filled histogram) or mouse IgG2a, κ PE isotype control (open histogram).
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362603 100 tests 335 CHF
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Description

This antibody was raised against a linear 8XHis tag. It reacts with His-tagged protein with C, N or internal 6XHis tags or longer. It can be used for western blotting to detect linear His repeats (6XHis or more). It can also be used for ICFC and ICC staining of His-tagged proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, All species
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH-conjugated His-tag peptide.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

This antibody was raised against a linear 8X His tag. It reacts with proteins displaying repeats of 6 Histidines (His6) or more in either internal or C and N terminal regions. It is suitable for ICFC.

Clone J095G46 has been verified for immunocytochemistry (ICC).

Product Citations
  1. Tasker C, et al. 2021. AAPS J. 23:26. PubMed
  2. Li Z, et al. 2019. Cell Mol Immunol. 0.897222222. PubMed
  3. Liu L, et al. 2022. Sci Transl Med. 14:eabn6859. PubMed
  4. Gall CLM, et al. 2023. Bio Protoc. 13:e4613. PubMed
  5. Lubin JH, et al. 2023. JCI Insight. . PubMed
  6. Golubovskaya V, et al. 2023. Cancers (Basel). 15:. PubMed
  7. Huang J, et al. 2021. Immunity. 54(4):673-686.e4. PubMed
  8. Wei C, et al. 2020. Nat Metab. 1.049305556. PubMed
  9. Le Gall CM, et al. 2021. Bioconjug Chem. 32:301. PubMed
  10. Yang J, et al. 2020. Nature. 586:572. PubMed
  11. Zhu QY, et al. 2021. Nat Commun. 12:6624. PubMed
  12. Tong P, et al. 2021. Cell. :. PubMed
  13. Huang HC, et al. 2021. EBioMedicine. 74:103712. PubMed
  14. Scheu AHA, et al. 2021. Nat Commun. 0.997916667. PubMed
  15. Pivniouk V, et al. 2022. J Allergy Clin Immunol. 149:923. PubMed
  16. Jandke A, et al. 2020. Nat Commun. 3.075694444. PubMed
  17. Long Y, et al. 2021. Biochem Biophys Res Commun. 571:152. PubMed
  18. van der Schoot JMS, et al. 2019. Sci Adv. 5:eaaw1822. PubMed
  19. Sokal A, et al. 2021. Immunity. S1074-7613:00396. PubMed
  20. Alberts A, et al. 2020. Front Immunol. 11:596103. PubMed
  21. Wang R, et al. 2021. Immunity. 54:1611. PubMed
  22. Zhang M, et al. 2022. iScience. 25:104490. PubMed
RRID
AB_2563634 (BioLegend Cat. No. 362603)

Antigen Details

Structure
6 repeat His amino acid
Function
Epitope tag
Biology Area
Cell Biology, Immunology
Antigen References
  1. Zhao C, et al. 2010. Anal Biochem. 399:237
  2. Salichs E, et al. 2009. PLoS Genet. 5:e1000397
  3. Terpe K. 2003. Appl. Microbiol. Biotechnol. 60:523
  4. Lindner, P, et al. 1997. Biotechniques. 22:140
  5. Burritt JB, et al. 1995. J. Biol. Chem. 270:16974.
  6. Taylor RM, et al. 2007. Methods Mol. Biol. 412:429.
Gene ID
NA
UniProt
View information about His Tag on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 08.19.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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