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PE anti-Histone H3 Phospho (Ser10) Antibody

Pricing & Availability
Clone
11D8 (See other available formats)
Regulatory Status
RUO
Other Names
Histone-H3, H3
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
11D8_PE_Histone_H3_Phospho_Antibody_ICFC_120414
HeLa cells were treated with 20 µM of Nocodazole for 24 hours, then fixed and permeabilized with 70% ethanol, and then stained with Histone H3 Phospho-Ser10 (clone 11D8) PE (filled histogram) or mouse IgG2b, κ PE isotype control (open histogram).
  • 11D8_PE_Histone_H3_Phospho_Antibody_ICFC_120414
    HeLa cells were treated with 20 µM of Nocodazole for 24 hours, then fixed and permeabilized with 70% ethanol, and then stained with Histone H3 Phospho-Ser10 (clone 11D8) PE (filled histogram) or mouse IgG2b, κ PE isotype control (open histogram).
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650807 25 tests 183 CHF
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650808 100 tests 414 CHF
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Description

Histone H3 is phosphorylated at serine 10 during mitosis and is found to be involved in transcriptional activation, chromatin decondensation, and chromosome compaction during cell division, by the action of Aurora kinase and NIMA kinases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Modified synthetic peptide conjugated to KLH
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The histone H3 pS10 antibody recognizes phosphorylation of human H3 protein at Ser10 residue and has been shown to be useful for Western blotting.

Product Citations
  1. Garcia-Fabiani MB, et al. 2020. STAR Protoc. :1. PubMed
  2. Gao H, et al. 2021. Cells. 10:. PubMed
  3. Jin J, et al. 2018. Neoplasia. 20:478. PubMed
RRID
AB_2564562 (BioLegend Cat. No. 650807)
AB_2564562 (BioLegend Cat. No. 650808)

Antigen Details

Structure
H3 is part of the nucleosome, comprised of an octameric complex with H2A, H2B, and H4 proteins.
Distribution

Nucleus

Function
H3 is a core component of the nucleosome that serves to wrap and compact DNA into chromatin. Histones, therefore, limit the accessibility of DNA, providing mechanisms for transcription regulation, DNA repair and replication, and chromosomal stability.
Interaction
Two molecules of H3 form a heterotetramer with two molecules of H4.
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, DNA Repair/Replication, Transcription Factors
Molecular Family
Phospho-Proteins
Antigen References

1. Choi HS, et al. 2005. J. Biol. Chem. 280:13545.
2. Goto H, et al. 2002. Genes Cells 7:11.
3. Garcia BA, et al. 2005. Biochemistry 44:13202.
4. Hans F, et al. 2001. Oncogene 20:3021.

Regulation
H3 is regulated by acetylation, methylation, citrullination, phosphorylation, and ubiquitination.
Gene ID
8290 View all products for this Gene ID
UniProt
View information about Histone on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 12.08.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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