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PE anti-HMOX1 Antibody

Pricing & Availability
Clone
W19398C (See other available formats)
Regulatory Status
RUO
Other Names
Heme Oxygenase 1, HO-1, Heat Shock Protein, 32-KD
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W19398C_PE_HOX1_ICFC_061223
HeLa cells untreated (low expression control, open histogram), or treated with CoCl2 (200 µM) (positive control, filled histogram) were fixed and permeabilized using Cyto-Fast™ Fix/Perm Buffer set (Cat. No. 426803) and intracellularly stained with PE anti-HMOX1 (clone W19398C), or PE rat IgG2a, κ isotype control (open histogram, dashed line) (representative for both untreated and treated HeLa cells) (Cat. No. 400507).
  • W19398C_PE_HOX1_ICFC_061223
    HeLa cells untreated (low expression control, open histogram), or treated with CoCl2 (200 µM) (positive control, filled histogram) were fixed and permeabilized using Cyto-Fast™ Fix/Perm Buffer set (Cat. No. 426803) and intracellularly stained with PE anti-HMOX1 (clone W19398C), or PE rat IgG2a, κ isotype control (open histogram, dashed line) (representative for both untreated and treated HeLa cells) (Cat. No. 400507).
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934103 25 tests 194 CHF
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934104 100 tests 382 CHF
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Description

HMOX1 is a member of the heme oxygenase family. Unlike its constitutive counterpart, it is an inducible enzyme triggered by external stimuli such as nitric oxide, growth factors, heavy metals, UV radiation, phorbol esters, inflammatory cytokines, heme, etc. Transcription of HMOX1 in tissue has been shown to be a product of the nrf2/ARE pathway where an increase in its protein levels results in heme degradation. The tissue protective effects of HMOX1 are a result of the bioactive products produced after heme degradation: iron ions, carbon monoxide, and biliverdin which is converted to bilirubin via biliverdin reductase.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone was tested for ICC using three fix/perm methods (PFA/Triton-X, PFA/MeOH, MeOH only), the best method was the use of PFA/Triton X-100.

In the CoCl2 treated cells for ICC, staining was seen in less than 5% of cells.

Clone was tested for ICFC using cytokine fix/perm method only (based on ICC results). 

 

RRID
AB_2941636 (BioLegend Cat. No. 934103)
AB_2941636 (BioLegend Cat. No. 934104)

Antigen Details

Distribution

Cytosol/Endoplasmic reticulum

Biology Area
Cell Biology
Molecular Family
Enzymes and Regulators
Antigen References

1. Yang B, et al. 2018. Toxicol Appl Pharmacol. 355:189-197
2. Chora A, et al. 2007. J Clin Invest. 117(2):438-47
3. Harding H, et al. 2003. Mol Cell. 11(3):619-33.

Gene ID
3162 View all products for this Gene ID
UniProt
View information about HMOX1 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 06.12.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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