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PE anti-human CD129 (IL-9 R) Antibody

Pricing & Availability
Clone
AH9R7 (See other available formats)
Regulatory Status
RUO
Other Names
CD129, IL-9R
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
CD129_AH9R7_092806
Human T lymphoblastic leukemia cell line HuT-78 stained with AH9R7 PE
  • CD129_AH9R7_092806
    Human T lymphoblastic leukemia cell line HuT-78 stained with AH9R7 PE
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310403 25 tests 134 CHF
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310404 100 tests 304 CHF
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Description

CD129 is known as the 57 kD IL-9 receptor. It is a member of the hematopoietin receptor superfamily. Although the α-chain of the receptor binds IL-9 with high affinity, interaction with the γ-chain (CD132) of the IL-2 receptor is required for signaling. The IL-9 receptor is expressed at low levels on eosinophils, mast cells, macrophages, B lymphocytes, T lymphocytes, and erythroid progenitors. IL-9 receptor binding initiates STAT activation required for the proliferative and anti-apoptotic effects of this cytokine. In humans, signals from the IL-9 receptor appear to be critical for intrathymic T cell development. IL-9 binding has been shown to increase IL-5 receptor expression and promote survival in human eosinophils.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: inhibition the binding of IL-9 to the high affinity a-chain of the human IL-9 receptor1,2, and ELISA for detection of soluble IL-9R. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 310404) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)). The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 310408).

Application References

(PubMed link indicates BioLegend citation)
  1. De Smedt M, et al. 2000. J. Immunol. 164:1761.
  2. Pilette C, et al. 2002. J. Immunol. 168:4103.
  3. Demoulin JB, et al.1996. Mol. Cell. Biol. 16 (9):4710.
Product Citations
  1. Das S, et al. 2021. J Immunol. 206:2740. PubMed
  2. Beatson RE, et al. 2021. Cell Rep Med. 2:100473. PubMed
  3. Bal S, et al. 2016. Nat Immunol. 10.1038/ni.3444. PubMed
  4. Xiao M, et al. 2017. Blood. 129:3196. PubMed
RRID
AB_314817 (BioLegend Cat. No. 310403)
AB_314817 (BioLegend Cat. No. 310404)

Antigen Details

Structure
Cytokine receptor family, type I membrane protein or soluble, associate with common γ chain(CD132), 57 kD
Distribution

Eosinophils, mast, thymocytes, erythroid, myeloid progenitors, macrophages

Function
Activates STAT; critical for intrathymic T cell development
Ligand/Receptor
IL-9
Cell Type
Eosinophils, Erythrocytes, Hematopoietic stem and progenitors, Macrophages, Mast cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Demoulin JB, et al.1996. Mol. Cell. Biol. 16:4710.

Gene ID
3581 View all products for this Gene ID
UniProt
View information about CD129 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 08.17.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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