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PE anti-human CD143 (Angiotensin-converting enzyme) Antibody

Pricing & Availability
Clone
5-369 (See other available formats)
Regulatory Status
RUO
Other Names
ACE, kininase II, dipeptidyl dipeptidase 1, peptidase P, carboxycathepsin
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
5-369_PE_CD143_Antibody_101518
GM-CSF-stimulated human peripheral blood mononuclear cells (day-3) were stained with CD143 (clone 5-369) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram). Data shown was gated on CD14+ cell population
  • 5-369_PE_CD143_Antibody_101518
    GM-CSF-stimulated human peripheral blood mononuclear cells (day-3) were stained with CD143 (clone 5-369) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram). Data shown was gated on CD14+ cell population
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344204 100 tests 317 CHF
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Description

CD143 (ACE, kininase II, peptidyl dipeptidase 1, peptidase P, carboxycathepsin) is a 171 kD, type I, single chain transmembranal metallopeptidase, whose cofactor is zinc. Its main targets are angiotensin I and bradykinin, acting as a blood pressure regulator. CD143 is expressed in endothelial cells; varying amounts of CD143 have been reported in different epithelial cells. The activation of macrophages and histiocytes induces the expression of this molecule.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Tomellini E, et al. 2020. Cell Reports. 28(4):1063-1073.e5.. PubMed
RRID
AB_2222985 (BioLegend Cat. No. 344204)

Antigen Details

Structure
Single chain, type I transmembranal zinc metallopeptidase, 170 kD.
Distribution

Endothelial cells, some types of epithelial cells, activated macrophages and histiocytes.

Function
Regulates blood pressure by promoting vasoconstriction by the conversion of angiotensin I to angiotensin II and by the degradation of bradykinin, a vasodilator.
Ligand/Receptor
Angiotensin I, bradykinin.
Bioactivity
Blood pressure regulator.
Cell Type
Endothelial cells, Epithelial cells, Macrophages
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Nakamura T, et al. 2009. Int Heart J. 50:501.
2. Jayasooriya AP, et al. 2008. P. Natl. Acad. Sci. USA 105:6531.
3. Jokubaitis VJ, et al. 2008. Blood 111:4055.
4. Arndt PG, et al. 2006. J. Immunol. 177:7233.
5. Balyasnikova IV, et al. 2002. Biochem J. 362:585.

Gene ID
1636 View all products for this Gene ID
UniProt
View information about CD143 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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