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PE anti-human CD184 (CXCR4) Antibody

Pricing & Availability
Clone
12G5 (See other available formats)
Regulatory Status
RUO
Workshop
VII 70204
Other Names
CXCR4, Fusin
Isotype
Mouse IgG2a, κ
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Product Citations
publications
12G5
Human peripheral blood lymphocytes stained with 12G5 PE
  • 12G5
    Human peripheral blood lymphocytes stained with 12G5 PE
Compare all formats See PE spectral data
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306505 25 tests 129 CHF
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306506 100 tests 297 CHF
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Description

CD184, also known as fusin or CXCR4, is a 45 kD seven transmembrane G-protein-linked CXC chemokine receptor. CD184 is widely expressed on blood and tissue cells, including B and T cells, monocytes, macrophages, dendritic cells, granulocytes, megakaryocytes/platelets, lymphoid, myeloid precursor cells, endothelial cells, epithelial cells, astrocytes, and neurons, among other tissue cells. CD184 is the receptor for CXC chemokine SDF-1, mediates blood cell migration, and is involved in B lymphopoiesis and myelopoiesis, cardiogenesis, blood vessel formation, and cerebellar development. CXCR4 is also a coreceptor of X4 HIV-1 and an alternative receptor for some isolates of HIV-2.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon, Chimpanzee, Sooty Mangabey
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
CP-MAC-infected Sup-T1 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections11, immunocytochemistry3, immunofluorescence microscopy2,6, and blocking of CD4-independent infection by HIV-2 and CD4-dependent infection by some T cell-tropic isolates of HIV-14,5. Clone 12G5 may not be suitable for Western blotting.10 The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 306539 & 306540).

Application References

(PubMed link indicates BioLegend citation)
  1. McKnight A, et al. 1997. J. Virol. 71:1692.
  2. Endres MJ, et al. 1996. Cell 87:745. (Immunogen, IF)
  3. Volin MV, et al. 1998. Biochem. Biophys. Res. Commun. 242:46. (ICC)
  4. Berndt C, et al. 1998. P. Natl. Acad. Sci. USA 95:12556. (Block)
  5. Ullrich CK, et al. 2000. Blood 96:1438. (Block)
  6. Murga M, et al. 2005. Blood 105:1992. (IF)
  7. Thompson BD. 2007. J. Biol. Chem. 282:9547. (FC) PubMed
  8. Isnardi I, et al. 2010. Blood 115:5026. PubMed
  9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  10. Fischer T, et al. 2008. PLoS One 3:e4069.
  11. Schmid BC, et al. 2004. Breast Cancer Res. Treat. 84:247. (IHC)
Product Citations
  1. Corral-Jara KF, et al. 2021. Mol Biomed. 2:9. PubMed
  2. Costa MHG, et al. 2021. Biotechnol J. 16:e2000389. PubMed
  3. Ando M, et al. 2021. Cancer Res Commun. 1:41. PubMed
  4. Scala S, et al. 2023. Nat Commun. 14:3068. PubMed
  5. Liu J, et al. 2022. Front Neurol. 12:807646. PubMed
  6. Guo P, et al. 2012. Biomaterials. 33:8104. PubMed
  7. Wang B, et al. 2013. Carcinogenesis. 35:282. PubMed
  8. Blengio F, et al. 2013. Immunobiology. 218:76. PubMed
  9. Li H, et al. 2016. J Immunol. 196: 4064 - 4074. PubMed
  10. Yang W, et al. 2019. iScience. 19:450. PubMed
  11. Muir L, et al. 2018. Wellcome Open Res. 2:97. PubMed
  12. Olga Alekhina, Adriano Marchese 2016. J Biol Chem. 291(50):26083-26097. PubMed
  13. Yang C, et al. 2020. J Control Release. 326:324. PubMed
  14. Wu T, et al. 2022. Cell Death Dis. 13:624. PubMed
  15. Hou P, et al. 2015. Sci Rep. 5: 15577. PubMed
  16. Chittasupho C, Anuchapreeda S, and Sarisuta N. 2017. Eur J Pharm Biopharm. 10.1016/j.ejpb.2017.07.003. PubMed
  17. Schneider T, et al. 2015. Glycobiology. 25: 812-824. PubMed
  18. Mandl M, et al. 2014. PLoS One. 9:112140. PubMed
  19. Mair B et al. 2019. Cell reports. 27(2):599-615 . PubMed
  20. Adikrisna R, et al. 2012. Gastroenterology. 143:234. PubMed
  21. Gurevich I, et al. 2020. Biol Open. . PubMed
  22. Han W, et al. 2015. Stem Cell Dev. 24: 2536-2546. PubMed
  23. Idorn M, et al. 2018. Oncoimmunology. 7:e1412029. PubMed
  24. Magri G et al. 2017. Immunity. 47(1):118-134 . PubMed
  25. Gurevich I, et al. 2020. Biol Open. . PubMed
  26. Yuan Z, et al. 2016. Cytotherapy. 18: 860-869. PubMed
  27. Houtsma R, et al. 2021. STAR Protoc. 2:100864. PubMed
  28. Jung Y, et al. 2018. Cancer Res. 78:2026. PubMed
  29. Suan D et al. 2017. Immunity. 47(6):1142-1153 . PubMed
  30. Hartmann T, et al. 2008. J Leukoc Biol. 84:1130. PubMed
  31. subtypes N 2015. Nat Commun. 6: 10183. PubMed
  32. Chen HJ, et al. 2019. J Exp Med. 216:674. PubMed
  33. Xu J, et al. 2017. Clin Cancer Res. 23:4482. PubMed
  34. Zhang B, et al. 2021. Nat Commun. 12:1714. PubMed
  35. Yin S, et al. 2015. Sci Rep. 5: 14432. PubMed
  36. Nevins AM, et al. 2017. Methods Mol Biol. 1722:151. PubMed
  37. Subramaniam N, et al. 2021. Blood Adv. 5:1259. PubMed
  38. Smith N, et al. 2017. Nat Commun. 8:14253. PubMed
  39. Bettman N, et al. 2015. Blood Cells Mol Dis. 55: 180-186. PubMed
  40. Zhang Y et al. 2018. Cell stem cell. 23(4):516-529 . PubMed
  41. He S, et al. 2021. iScience. 24:103305. PubMed
RRID
AB_314611 (BioLegend Cat. No. 306505)
AB_314611 (BioLegend Cat. No. 306506)

Antigen Details

Structure
Rhodopsin family, G-protein linked seven transmembrane glycoprotein, 45 kD
Distribution

T cells and B cells, dendritic cells, monocytes, granulocytes, hematopoietic progenitors, endothelial cells

Function
B lymphopoiesis and myelopoiesis, cardiogenesis, blood vessel formation, cerebellar development
Ligand/Receptor
SDF-1 receptor, coreceptor for X4 HIV-1
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Monocytes, Neural Stem Cells, T cells, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Berger E, et al. 1999. Annu. Rev. Immunol. 17:657.
2. Loetscher P, et al. 2000. Adv. Immunol. 74:127.
3. Murphy P, et al. 2000. Pharmacol. Rev. 52:145.

Gene ID
7852 View all products for this Gene ID
UniProt
View information about CD184 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Go To Top Version: 6    Revision Date: 01.05.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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