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PE anti-human CD202b (Tie2/Tek) Antibody

Pricing & Availability
Clone
A19069E (See other available formats)
Regulatory Status
RUO
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
A19069E_PE_CD202b_FC_052824
Human endothelial cell line, HUVEC, were stained with anti-human CD202b (Tie2/Tek) (clone A19069E) PE (filled histogram) or mouse IgG2a, κ PE isotype control (open histogram).
  • A19069E_PE_CD202b_FC_052824
    Human endothelial cell line, HUVEC, were stained with anti-human CD202b (Tie2/Tek) (clone A19069E) PE (filled histogram) or mouse IgG2a, κ PE isotype control (open histogram).
See PE spectral data
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390553 25 tests 115 CHF
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390554 100 tests 260 CHF
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Description

CD202b, also known as Tie2 or TEK, is a 145 kD type I transmembrane protein. It is a member of the receptor tyrosine kinase (RTK) family of proteins and is expressed by endothelial cells and their progenitors, quiescent hematopoietic stem cells (HSCs), and a subpopulation of monocytes. Angiopoietin-1 (Ang-1) is an activator of CD202b to promote, maintain, and stabilize mature vessels and to maintain HSCs in quiescent state. Ang-2 is another ligand of CD202b, which is involved in postnatal angiogenesis and in antagonizing the effects of Ang-1. Tie2 binds to Ang-4 as well.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant extracellular domain of human Tie2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone A19069E partially block the staining of reference clone 33.1

​Recombinant protein completely blocks the staining of test clone A19069E

Antigen Details

Structure
145 kD type I transmembrane protein, receptor tyrosine kinase (RTK) family
Distribution

Endothelial cells, subsets of hematopoietic stem cells and and a subset of monocytes

Function
Angiogenesis, hemotopoiesis, monocytes chemotaxis
Interaction
Endothelial cells, hematopoietic stem and progenitors, monocytes
Ligand/Receptor
Angiopoietin-1 (Ang-1), Ang-2, and Ang-4
Cell Type
Endothelial cells, Hematopoietic stem and progenitors, Monocytes
Biology Area
Angiogenesis, Cell Biology, Immunology
Molecular Family
CD Molecules
Antigen References
  1. De Palma M, et al. 2005. Cancer Cell. 8:211-26.
  2. Shaw JP, et al. 2004. Blood Cells Mol Dis. 32:168-75.
  3. Hsu HC, et al. 2000. Blood. 96:3757-62.
  4. Arai F, et al. 2004. Cell. 118:149-61.
Gene ID
7010 View all products for this Gene ID
UniProt
View information about CD202b on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 05.28.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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