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PE anti-human CD218a (IL-18Rα) Antibody

Pricing & Availability
Clone
H44 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
IL-18 receptor alpha chain, IL-18 receptor 1, IL-18 receptor related protein, CDw218a
Isotype
Mouse IgG1, κ
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Product Citations
publications
H44_PE_072007
Human peripheral blood lymphocytes stained with H44 PE
  • H44_PE_072007
    Human peripheral blood lymphocytes stained with H44 PE
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313808 100 tests 335 CHF
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Description

IL-18 receptor is composed of an α and a β subunit that combine to form a high affinity receptor for IL-18. IL-18 receptor α chain, also known as CDw218a, is a 75-80 kD type I transmembrane protein. It is expressed on NK cells, neutrophils, endothelial cells, and subsets of T and B cells. The expression of CDw218a on lymphocytes is upregulated after activation. The interaction of IL-18 and IL-18 receptor has been reported to be implicated in promotion of Th1 cytokine production and atherogenesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human NK cell line NK0 constitutively expressing IL-18 receptors
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

 The H44 antibody is specific for IL-18 receptor a chain. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and neutralization1.

Application References

(PubMed link indicates BioLegend citation)
  1. Kitasato Y, et al. 2004. Am. J. Respir. Cell Mol. Biol. 31:619. (IHC)
  2. Vermot-Desroches C, et al. 2005. Cell Immunol. 236:101. (FC)
Product Citations
  1. Hagel J, et al. 2021. J Immunol. 206:3073. PubMed
  2. Spallanzani R, et al. 2015. J Immunol. 195: 2141-2148. PubMed
  3. Huang Y, et al. 2020. FASEB J. 34:1768. PubMed
  4. Hofbauer D, et al. 2021. Immunity. 54(8):1772-1787.e9. PubMed
  5. Halkias J, et al. 2019. J Clin Invest. 130:3562. PubMed
RRID
AB_345314 (BioLegend Cat. No. 313808)

Antigen Details

Structure
Type I membrane protein, IL-1 receptor family, approximately 75-80 kD
Distribution

Expressed on NK cells and neutrophils, IL-12 activated T and B cells, endothelial cells, and smooth muscle cells; highly expressed on Hodgkin's disease cell lines

Function
Binds IL-18 with low affinity; high affinity binding site formed with IL-18Rβ
Ligand/Receptor
IL-18
Cell Type
B cells, Endothelial cells, Neutrophils, NK cells, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Torigoe K, et al. 1997. J. Biol. Chem. 272:25737.
2. Gerdes N, et al. 2002. J. Exp. Med. 195:245.
3. Airoldi I, et al. 2000. J. Immunol. 165:6880.

Gene ID
8809 View all products for this Gene ID
UniProt
View information about CD218a on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 3    Revision Date: 10.31.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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