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PE anti-human CD257 (BAFF, BLYS) Antibody

Pricing & Availability
Clone
1D6 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
BAFF, BLYS, TNFSF13b, B cell activating factor, tumor necrosis factor ligand superfamily 13B, B lymphocyte stimulator (BLYS), TNF and APOL-related leukocyte expressed ligand 1 (TALL1), TNF homolog that activates apoptosis, NF-κB and JNK (THANK)
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1D6_PE_CD257_Antibody_FC_051115
Human CD257-transfected bovine coronary artery endothelial cell line (B300) was stained with anti-human CD257 (clone 1D6) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
  • 1D6_PE_CD257_Antibody_FC_051115
    Human CD257-transfected bovine coronary artery endothelial cell line (B300) was stained with anti-human CD257 (clone 1D6) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
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366505 25 tests 165 CHF
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366506 100 tests 283 CHF
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Description

BAFF, also known as B cell activating factor, tumor necrosis factor ligand superfamily 13B, B lymphocyte stimulator (BLYS), TNF and APOL-related leukocyte expressed ligand 1 (TALL1), and TNF homolog, activates apoptosis, NF-κB, and JNK (THANK). BAFF is a type II transmembrane protein and is a member of the tumor necrosis factor ligand superfamily. BAFF can be proteolytically cleaved to form a soluble protein. BAFF, like other TNF ligand family members, forms homotrimers. The predicted molecular weight of a BAFF monomer is approximately 31 kD. BAFF is expressed on peripheral blood B and T lymphocytes, monocytes, macrophages, and dendritic cells. It is upregulated by IFN-γ and downregulated by PMA/ionomycin treatment.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human CD257 transfected murine cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Hase H, et al. 2004. Blood 6:2257. (IHC)
RRID
AB_2565708 (BioLegend Cat. No. 366505)
AB_2565708 (BioLegend Cat. No. 366506)

Antigen Details

Structure
Type II transmembrane protein and is a member of the tumor necrosis factor ligand superfamily. It can be proteolytically cleaved to form a soluble protein and it forms homotrimers. The predicted molecular weight of monomer is approximately 31 kD.
Distribution

Peripheral blood B and T lymphocytes, monocytes, macrophages, and dendritic cells. Upregulated by IFN-γ and is downregulated by PMA/ionomycin treatment.

Function
Stimulates B and T cell immunity, regulates humoral immunity by binding TACI and BCMA receptors, and prevents apoptosis and promotes B cell survival by binding the BAFF-specific receptor (BAFFR/BR3).
Ligand/Receptor
BAFF binds to TACI (TNFRSF13B) and BCMA (TNFRSF17) as well as the APRIL ligand. BAFF also binds to the BAFF-specific receptor (BAFFR/BR3).
Cell Type
B cells, Dendritic cells, Macrophages, Monocytes, T cells
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Signal Transduction, Stem Cells
Molecular Family
CD Molecules, Cytokines/Chemokines, Growth Factors, Soluble Receptors
Antigen References

1. Schneider P, et al. 1999. J. Exp. Med. 189:1747.
2. Moore PA, et al. 1999. Science 285:260.
3. Shu HB, et al. 1999. J. Leuko. Biol. 65:680.
4. Yu G, et al. 2000. Nat. Immunol. 1:252.
5. Oren DA, et al. 2002. Nat. Struct. Biol. 9:288.

Gene ID
10673 View all products for this Gene ID
UniProt
View information about CD257 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 05.12.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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