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PE anti-human CD318 (CDCP1) Antibody

Pricing & Availability
Clone
CUB1 (See other available formats)
Regulatory Status
RUO
Workshop
HLDA8
Other Names
CDCP1, CUB domain containing protein 1, SIMA135
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
CUB1_PE_042307
HT-29 cells (human colon carcinoma) were stained with PE anti-human CD318 (clone CUB1) (filled histogram) or mouse IgG2b, κ PE isotype control (open histogram).
  • CUB1_PE_042307
    HT-29 cells (human colon carcinoma) were stained with PE anti-human CD318 (clone CUB1) (filled histogram) or mouse IgG2b, κ PE isotype control (open histogram).
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Cat # Size Price Quantity Check Availability Save
324017 25 tests 134 CHF
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324006 100 tests 292 CHF
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Description

The CUB1 antibody recognizes CD318 also known as CDCP1, CUB domain containing protein 1, and SIMA135. CD318 is a transmembrane protein containing three CUB domains with a predicted molecular weight of approximately 93 kD. Alternately spliced isoforms have also been reported. CD318 is a marker for immature hematopoietic progenitor cell subsets and is also expressed in mesenchymal stem cells and neural progenitor cell populations. This marker is also widely distributed in a variety of cell types including keratinocytes, skeletal muscle, colon, kidney, lung, small intestine. CD318 is overexpressed in colon and lung tumors and expression level has been shown to correlate with metastatic ability of carcinomas. CD318 interacts with plasminogen, matriptase, and c-Src (phosphorylates residue Pyr734). The function of CD318 is not well understood although it is thought to be involved in early hematopoiesis. The CUB1 antibody has been shown to be useful for flow cytometry.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NIH-3T3 cells transfected with human CDCP1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Bühring HJ, et al. 2004. Stem Cells 22:334-343.
Product Citations
  1. Miksiunas R, et al. 2022. Cells. 11:. PubMed
  2. Tomellini E, et al. 2020. Cell Reports. 28(4):1063-1073.e5.. PubMed
RRID
AB_756056 (BioLegend Cat. No. 324017)
AB_756056 (BioLegend Cat. No. 324006)

Antigen Details

Structure
Transmembrane protein containing three CUB domains, predicted molecular weight approximately 93 kD. Alternately spliced isoforms have been reported
Distribution

Marker for immature hematopoietic progenitor cell subsets; also expressed in mesenchymal stem cells and neural progenitor cell populations. Widely distributed in a variety of cell types including keratinocytes, skeletal muscle, colon, kidney, lung, small

Function
Unknown, thought to be involved on early hematopoiesis
Interaction
Plasminogen, matriptase, c-Src
Modification
Phosphorylation (Y734) by c-Src; regulated by adhesion or plasmin in epithelial cells
Cell Type
Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Neural Stem Cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Brown TA, et al. 2004. J. Biol. Chem. 279:14772.
2. Conze T, et al. 2003. Ann. N.Y. Acad. Sci. 996:222.
3. Hooper JD, et al. 2003. Oncogene 22:1783.

Gene ID
64866 View all products for this Gene ID
UniProt
View information about CD318 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 04.19.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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