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PE anti-human CD34 Antibody

Pricing & Availability
Clone
S20016E (See other available formats)
Regulatory Status
RUO
Other Names
Hematopoietic progenitor cell antigen CD34
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
S20016E_PE_-CD34_FC_052623
Human peripheral blood mononuclear cells were stained with anti-human CD45 Brilliant Violet 421™ and anti-human CD34 (clone S20016E) PE (left) or mouse IgG2a, κ PE isotype control (right). Data shown was gated on CD14 - cell population.
  • S20016E_PE_-CD34_FC_052623
    Human peripheral blood mononuclear cells were stained with anti-human CD45 Brilliant Violet 421™ and anti-human CD34 (clone S20016E) PE (left) or mouse IgG2a, κ PE isotype control (right). Data shown was gated on CD14 - cell population.
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378603 25 tests 125 CHF
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378604 100 tests 285 CHF
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Description

CD34, also known as gp105-120, is a type I monomeric sialomucin-like glycophosphoprotein with an approximate molecular weight of 105-120 kD. Since it is selectively expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nervous tissue, CD34 is a commonly used marker to identify human hematopoietic stem/progenitor cells. According to the differential sensitivity to enzymatic cleavage, four groups of epitopes of CD34 have been described. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands. About 40% of acute myeloid leukemias and 65% of pre-B acute lymphoblastic leukemias express CD34, whereas only 1-5% of acute T-lymphoid leukemias are CD34 positive.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human CD34-transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The S20016E antibody recognizes a class III group epitope, which is resistant to sialidase/glycolyprotease treatment.

RRID
AB_2941552 (BioLegend Cat. No. 378603)
AB_2941552 (BioLegend Cat. No. 378604)

Antigen Details

Structure
Single-pass type I membrane protein
Distribution

Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts

Function
Cell adhesion
Interaction
L-selectin, CRKL
Ligand/Receptor
L-selectin, E-selectin
Cell Type
Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family
CD Molecules
Antigen References
  1. Simmons DL, et al. 1992. Journal of Immunology. 148: 267-71.
  2. Krause DS, et al. 1996. Blood. 84: 1-13.
  3. Sidney LE, et al. 2014. Stem Cells. 32:1380-9. 

 

Gene ID
947 View all products for this Gene ID
UniProt
View information about CD34 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

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Go To Top Version: 1    Revision Date: 05.26.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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