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PE anti-human CD35 Antibody

Pricing & Availability
Clone
9H3 (See other available formats)
Regulatory Status
RUO
Other Names
C3b/C4b receptor, complement receptor type 1 (CR1)
Isotype
Mouse IgG1, κ
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Product Citations
publications
9H3_PE_CD35_Antibody_031521.png
Human peripheral blood lymphocytes were stained with CD20 PE/Cyanine7 and CD35 (clone 9H3) PE (left) or mouse IgG1, ĸ PE isotype control (right).
  • 9H3_PE_CD35_Antibody_031521.png
    Human peripheral blood lymphocytes were stained with CD20 PE/Cyanine7 and CD35 (clone 9H3) PE (left) or mouse IgG1, ĸ PE isotype control (right).
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332403 25 tests 122 CHF
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332404 100 tests 268 CHF
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Description

CD35 is a type I single chain of glycoprotein, also known as C3b/C4b receptor, Complement Receptor type 1 or CR1. Four molecular weight allotypes (160kD, 190kD, 220kD, and 250kD) have been described. CD35 is expressed on granulocytes, monocytes, B cells, erythrocytes, and follicular dendritic cells, as well as subsets of NK and T cells. CD35 binds complement C3b, C4b, or iC3, and iC4, and plays important roles in both innate and adoptive immune response via mediating phagocytosis by granulocytes and monocytes. CD35 has also been reported to inhibit T-cell proliferation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. LaSalle TJ, et al. 2022. Cell Rep Med. 3:100779. PubMed
RRID
AB_2890765 (BioLegend Cat. No. 332403)
AB_2890765 (BioLegend Cat. No. 332404)

Antigen Details

Distribution

B cells, Dendritic cells, Erythrocytes, Granulocytes, Monocytes, NK cells, T cells

Function
Adhesion, mediate phagocytosis, inhibit T-cell proliferation
Ligand/Receptor
C3b, iC3, C4b, iC4
Cell Type
B cells, Dendritic cells, Erythrocytes, Granulocytes, Monocytes, NK cells, T cells
Biology Area
Cell Biology, Complement, Immunology, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Vorup-Jensen et al. 2018. Front Immunol. 9:2716. 
2. Ogembo et al. 2013. Cell Rep. 3(2):371-85.

Gene ID
1378 View all products for this Gene ID
UniProt
View information about CD35 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 03.15.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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