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PE anti-human CD56 (NCAM) Antibody

Pricing & Availability
Clone
W22097A (See other available formats)
Regulatory Status
RUO
Other Names
Leu-19, NKH1, NCAM-1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W22097A_PE_CD56_FC_061824
Human peripheral blood lymphocytes were stained with anti-human CD16 (clone 3G8) APC and anti-human CD56 (NCAM) (clone W22097A) PE (left) or rat IgG2a, κ PE isotype control (right).
  • W22097A_PE_CD56_FC_061824
    Human peripheral blood lymphocytes were stained with anti-human CD16 (clone 3G8) APC and anti-human CD56 (NCAM) (clone W22097A) PE (left) or rat IgG2a, κ PE isotype control (right).
See PE spectral data
Cat # Size Price Quantity Check Availability Save
343753 25 tests 100 CHF
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343754 100 tests 260 CHF
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Description

CD56, also known as NCAM (neural cell adhesion molecule), Leu-19, or NKH1, is a single transmembrane glycoprotein. It is a member of the Ig superfamily. The 140 kD isoform is expressed on NK and NKT cells. CD56 is also expressed in the brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56. CD56 plays a role in homophilic and heterophilic adhesion via binding to itself or heparan sulfate.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mammalian-derived recombinant protein aa 20-718
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Flow cytometry testing showed that clone W22097A can block the binding of clone HCD56 and W22098E on target cells.

Flow cytometry testing showed that clone W22097A does not affect the binding of clone MEM-188, 5.1H11 and W22098A on target cells.

RRID
AB_3662242 (BioLegend Cat. No. 343753)
AB_3662242 (BioLegend Cat. No. 343754)

Antigen Details

Structure
Ig superfamily, single transmembrane or GPI-anchored glycoprotein
Distribution

NK cells, T subset, neural tissue, some LGL and myeloid leukemias

Function
Adhesion
Ligand/Receptor
Heparan sulfate
Cell Type
Leukemia, Neurons, NK cells, NKT cells
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Lanier L, et al. 1991. J Immunol. 146:4421-6.
  2. Hemperly J, et al. 1990. J Mol Neurosci. 2:71-8.
  3. Cremer H, et al. 1994. Nature. 367:455-9.
Gene ID
4684 View all products for this Gene ID
UniProt
View information about CD56 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 06.18.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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