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PE anti-human CD66 (CEACAM4/6) Antibody

Pricing & Availability
Clone
W20030D (See other available formats)
Regulatory Status
RUO
Other Names
CEACAM4, CEACAM6
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W20030D_PE_CD66_Antibody_053123
Human peripheral blood granulocytes were stained with anti-human CD66 (CEACAM4/6) (clone W20030D) PE (filled histogram) or rat IgG2b, κ PE isotype control (open histogram).
  • W20030D_PE_CD66_Antibody_053123
    Human peripheral blood granulocytes were stained with anti-human CD66 (CEACAM4/6) (clone W20030D) PE (filled histogram) or rat IgG2b, κ PE isotype control (open histogram).
See PE spectral data
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387703 25 tests 140 CHF
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387704 100 tests 275 CHF
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Description

CEACAM4, also known as CGM7, belongs to the immunoglobulin superfamily, specifically the carcinoembryonic antigen (CEA)-like subfamily. It is an orphan receptor that serves a phagocytic function in human granulocytes.

CD66c, also known as CEACAM6, is one of the splice variants of CD66. It is expressed on endothelial, epithelial cells, neutrophils, monocytes, and myeloma and cancer cells, such as B-cell acute lymphoblastic lymphomas. CD66 expression level can be induced on T cells, B cells, and CD16-negative NK cells. Like the other CD66 splice variants, CD66c is involved in cell adhesion and growth, neutrophil activation, and signaling. It plays a role in hemophilic and heterophilic adhesion.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant protein, aa 35-320
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone W20030D binds both CEACAM4 and CEACAM6.

RRID
AB_2941578 (BioLegend Cat. No. 387703)
AB_2941578 (BioLegend Cat. No. 387704)

Antigen Details

Structure
Ig superfamily, CEA antigen group, GPI-linked glycoprotein
Distribution

Granulocytes, Monocytes, epithelial cells

Function
Cell adhesion, neutrophil activation, homophilic and heterophilic adhesion
Interaction
Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM5 and CEACAM8
Ligand/Receptor
CD66c, CD66a-e, CD62E and Galectins
Molecular Family
Adhesion Molecules
Antigen References
  1. Delgado Tascón J, et al. 2015. J Leukoc Biol. 97(3):521-31.
  2. Duxbury MS, et al. 2004. Oncogene. 23:465–73.
  3. Oikawa S, et al. 1991. J Biol Chem. 266:7995–8001.
  4. Noworolska A, et al. 1986. Cancer Detect Prev. 9:365–71.
  5. Wu S, et al. 2021. Transl Onc. 14:101057.
Gene ID
1089 View all products for this Gene ID 4680 View all products for this Gene ID
UniProt
View information about CD66 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 05.31.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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