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PE anti-human cdc2 Phospho (Thr14) Antibody

Pricing & Availability
Clone
A20004B (See other available formats)
Regulatory Status
RUO
Other Names
Cyclin Dependent Kinase 1, Cdk1, CDC28A, P34CDC2, P34 Protein Kinase
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
a_A20004B_PE_cdc2_1_030923
HeLa cells treated with 4mM Hydroxyurea for 20 hours were fixed with Fixation Buffer (Cat. No. 420801), permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401), and then intracellularly stained with PE anti-human cdc2 Phospho (Thr14) (clone A20004B) (filled histogram, positive control) or with PE mouse IgG2b, κ isotype control (Cat. No. 400312) (open histogram, dashed line, negative control).
  • a_A20004B_PE_cdc2_1_030923
    HeLa cells treated with 4mM Hydroxyurea for 20 hours were fixed with Fixation Buffer (Cat. No. 420801), permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401), and then intracellularly stained with PE anti-human cdc2 Phospho (Thr14) (clone A20004B) (filled histogram, positive control) or with PE mouse IgG2b, κ isotype control (Cat. No. 400312) (open histogram, dashed line, negative control).
  • b_A20004B_PE_cdc2_2_030923
    Hydroxyurea-treated HeLa cells (filled histogram, positive control) or untreated HeLa cells (open histogram, solid line, negative control) were fixed with Fixation Buffer (Cat. No. 420801), permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401), and intracellularly stained with PE anti-human cdc2 Phospho (Thr14) (clone A20004B).
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947403 25 tests 194 CHF
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947404 100 tests 382 CHF
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Description

Cyclin-dependent kinase 1 (Cdk1), also known as cell division control protein 2 (cdc2), is a key regulatory kinase essential for cell growth, proliferation, cell cycle progression, and early embryonic development. It is responsible for driving cells through G2 phase into M phase. Prior to mitosis, cdc2, along with its binding partner cyclin B1, exists in an inactive state when phosphorylated on Thr14 and Tyr15 by PKMYT1 (MYT1) and WEE1. This B1-cdc2 complex is activated during G2 and early mitosis by cdc25-mediated dephosphorylation. During mitosis, cdc2 inhibits DNA re-replication and subsequent polyploidy and cell senescence through the sequestering of cyclin A2 and suppression of the formation of CDK2-cyclin A complexes. cdc2 can function as a substitute for other cdc family members and drive the progression of the cell cycle in the event of their loss. cdc2 functions as a positive regulator of global translation, protein synthesis, and genome stability and participates in DNA damage repair, checkpoint activation, and DNA replication fork progression. It is overexpressed in several cancers including liver, breast, and colorectal cancers and has been implicated in the promotion of tumorigenesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide corresponding to human cdc2 phosphorylated at threonine 14
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

This clone is predicted to react with mouse cdc2 when phosphorylated at threonine 14 due to complete sequence homology between immunizing peptide and the cdc2 mouse orthologue.

This clone was tested for ICC using lambda protein phosphatase analysis and HeLa cells fixed with methanol or 4% PFA followed by permeabilization with either methanol or Triton X-100. Only PFA fixation followed by methanol permeabilization produced phospho-specific staining of cdc2.

RRID
AB_2936757 (BioLegend Cat. No. 947403)
AB_2936757 (BioLegend Cat. No. 947404)

Antigen Details

Structure
cdc2 is a 297 amino acid protein with a predicted molecular weight of 34 kD.
Distribution

Ubiquitously expressed/ Mitochondria, nucleus, cytoskeleton, cytoplasm

Function
Serine/threonine-protein kinase/ Cell Cycle
Interaction
Cyclin B1, Cyclin A, PKMYT1 (MYT1)
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase
Antigen References
  1. Chow J, et al. 2011. Mol. Cell Biol. 31:1478-91.
  2. Diril M, et al. 2012. PNAS. 109:3826-31.
  3. Haneke K, et al. 2020. J. Cell. Biol. 219: e201906147.
  4. Liao H, et al. 2017. Oncotarget. 8:90662-73.
  5. Prevo R, et al. 2018. Cell Cycle. 17:1513-23.
Gene ID
983 View all products for this Gene ID
UniProt
View information about cdc2 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 03.09.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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