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PE anti-human HLA-DQ Antibody

Pricing & Availability
Clone
HLADQ1 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class II DQ monomorphic antigen, MHC II
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
HLADQ1_PE_111407
Human peripheral blood lymphocytes stained with HLADQ1 PE and overlayed with isotype control
  • HLADQ1_PE_111407
    Human peripheral blood lymphocytes stained with HLADQ1 PE and overlayed with isotype control
See PE spectral data
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318105 25 tests 122 CHF
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318106 100 tests 268 CHF
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Description

HLA-DQ is also known as MHC class II DQ monomorphic antigen. The major histocompatibility complex is composed of two heterodimeric glycoproteins (α and β chains) with apparent molecular weights of 27 and 32 kD. In contrast to other MHC class II molecules, both polypeptide chains of HLA-DQ are polymorphic, with the α chain showing an extremely high degree of polymorphism. HLA-DQ is expressed on B cells in the peripheral blood, and weakly expressed on activated T cells and some monocytes. HLA-DQ is absent on hematopoietic progenitors, resting T cells, erythrocytes, and platelets. HLA-DQ is expressed after HLA-DR and HLA-DP in hematopoietic development. HLA-DQ presents peptide fragments mainly from degraded intravesicular and extracellular proteins to CD4+ T lymphocytes. Specific alleles of HLA-DQ have been linked to the pathogenesis of several autoimmune diseases (including diabetes), both as a susceptibility and resistance factor depending on the particular polymorphism.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The antibody HLADQ1 reacts with HLA-DQ 4,5,6,7,8,9 but does not react with DQ2, DR or DP. Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded tissues.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leukocyte Typing IV. Oxford University Press. New York.
  2. Pazmandi K, et al. 2012. PLoS One. 7:e52085. PubMed
  3. Narita T, et al. 2014. J. Immunol. 192:940. PubMed
  4. Uhlenbrock F, et al. 2014. J Immunol. 193:1654. PubMed
  5. Kaushansky N, et al. 2015. J Biol Chem. 290:15260. PubMed
Product Citations
  1. Ivan Jelcic et al. 2018. Cell. 175(1):85-100 . PubMed
  2. Uhlenbrock F, et al. 2014. J Immunol. 193:1654. PubMed
  3. Ahmed R et al. 2019. Cell. 177(6):1583-1599 . PubMed
  4. Schreibelt G, et al. 2016. Clin Cancer Res. 22: 2155 - 2166. PubMed
  5. Pazmandi K, et al. 2012. PLoS One. 7:e52085. PubMed
  6. Junghans V, et al. 2020. J Cell Sci. 133:00:00. PubMed
  7. Jin Y et al. 2019. Nature communications. 10(1):391 . PubMed
  8. Pellegrini JM, et al. 2021. Sci Rep. 11:13559. PubMed
  9. Narita T, et al. 2014. J Immunol. 192:940. PubMed
  10. Crivello P, et al. 2019. J Immunol. 202:1895. PubMed
  11. Kaushansky N, et al. 2015. J Biol Chem. 290:15260. PubMed
  12. Song S, et al. 2021. Front Immunol. 12:705140. PubMed
RRID
AB_604127 (BioLegend Cat. No. 318105)
AB_604127 (BioLegend Cat. No. 318106)

Antigen Details

Structure
Major histocompatability complex composed of two heterodimeric glycoproteins (α and β chain) with apparent molecular weights of 27 and 32 kD. In contrast to other MHC class II molecules, both polypeptide chains of HLA-DQ are polymorphic.
Distribution

Expressed on B cells in the peripheral blood, weakly on activated T cells and some monocytes. Absent on hematopoietic progenitors, resting T cells, erythrocytes and platelets. Expressed after HLA-DR and HLA-DP in hematopoietic development.

Function
HLA-DQ presents peptide fragments mainly from degraded intravesicular and extracellular proteins to CD4+ lymphocytes. Specific alleles are linked to the pathogenesis of several autoimmune diseases.
Interaction
CD3/TCR, CD4
Cell Type
Thymocytes, T cells, Leukemia
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. So AK, et al. 1987. J. Immunol. 139:3506.
2. Gyllensten UB Erlih HA. 1989. Proc. Natl. Acad. Sci. USA 86:9986.
3. Sonderstrup G and McDevitt HO. 2001. J. Clin. Invest. 107:795.

Gene ID
3117 View all products for this Gene ID 3119 View all products for this Gene ID
UniProt
View information about HLA-DQ on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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