PE anti-human HLA-DRB1 Antibody anti-HLA-DRB1

Pricing & Availability

Clone: NFLD.D2 (See other available formats)
Regulatory Status: RUO
Other Names: SS1, DRB1, DRw10, HLA-DRB, HLA-DR1B
Isotype: Mouse IgG1, κ
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Product Citations: publications

NFLD.D2_PE_HLA-DRB1_Antibody_FC_1_051414.jpg — Human peripheral blood lymphoyctes were stained with CD20 APC and HLA-DRB1 (clone NFLD.D2, top) PE or mouse IgG1, κ PE isotype control (bottom).

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362303

25 tests

165 CHF

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362304

100 tests

384 CHF

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Description

HLA-DRB1, a HLA class II beta chain paralog, is a heterodimer membrane anchored α (DRA) and β chain (DRB). It plays an imporant role in extracellular protein-derived peptide presentation. It is expressed by antigen presenting cells (APC) such as B cells, dendritic cells, and macrophages. The β chain (composed of β1 and β2) is 26-28 kD and contains polymorphisms allowing for variability in peptide binding specificities. DRB1 is expressed by all individuals and its expression is five fold higher than DRB chains 3, 4, or 5. The peptide binding groove can accommodate peptides 10-30 residues long. Thirty-three different sub-types of HLA-DRB1*04 have been described. Of these, HLA-DRB1*04 is associated with an increased incidence of rheumatoid arthritis and is present on a subset of DRB1 molecules.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: L-cell transfectants HLA-DRB1*0401(11th IHW 8115), CFA
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone NFLD.D2 recognizes a shared epitope (DRB1*70-74:QKRAA and QRRAA) of HLA_DRB1*04.
RRID: AB_2563518 (BioLegend Cat. No. 362303)
AB_2563518 (BioLegend Cat. No. 362304)

Antigen Details

Structure: MHC class II cell surface receptor, 30 kD
Distribution: APCs
Function: Presentation of the peptide to T cells leads to activation or suppression of the immune system, which eventually leads to the immune response.
Interaction: CD4⁺ T cells
Cell Type: Antigen-presenting cells, B cells, Dendritic cells
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Scally SW, et al. 2013. J. Exp. Med. 210:2569.
2. Balnyte R, et al. 2013. BMC Neurol. 13:77.
3. Ranasinghe S, et al. 2013. Nat. Med. 19:930.
4. Foo JN, et al. 2013. Am. J. Hum. Genet. 93:167.
5. Balandraud N, et al. 2013. PLoS One. 8:e64108.
6. Sutton VR, et al. 1988. Hum. Immunol. 22:123.
7. Stunz LL, et al. 1989. J. Immunol. 143:3081.
Gene ID: 3123 View all products for this Gene ID
UniProt: View information about HLA-DRB1 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Related FAQs

What type of PE do you use in your conjugates?: We use R-PE in our conjugates.

Other Formats

View All HLA-DRB1 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human HLA-DRB1	NFLD.D2	FC
PE anti-human HLA-DRB1	NFLD.D2	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 06.16.2014

For Research Use Only. Not for diagnostic or therapeutic use.

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