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PE anti-human IDO1 Antibody

Pricing & Availability
Clone
W20099D (See other available formats)
Regulatory Status
RUO
Other Names
Indolamine 2,3-dioxygenase, Indole 2,3-dioxygenase, Indoleamine-pyrrole 2,3-dioxygenase
Isotype
Rat IgG2b, λ
Ave. Rating
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Product Citations
publications
A. W20099D_PE_IDO1_FC_082124
LPS stimulated (24 hours) human peripheral blood mononuclear cells were surface stained with anti-human CD11c (clone Bu15) APC. Cells were then fixed, permeabilized, and intracellularly stained with anti-human IDO1 (clone W20099D) PE (left), or rat IgG2b, λ PE isotype control (right).
  • A. W20099D_PE_IDO1_FC_082124
    LPS stimulated (24 hours) human peripheral blood mononuclear cells were surface stained with anti-human CD11c (clone Bu15) APC. Cells were then fixed, permeabilized, and intracellularly stained with anti-human IDO1 (clone W20099D) PE (left), or rat IgG2b, λ PE isotype control (right).
See PE spectral data
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335853 25 tests 125 CHF
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335854 100 tests 325 CHF
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Description

IDO1 is also known as Indolamine 2,3-dioxygenase, Indole 2,3-dioxygenase, and Indoleamine-pyrrole 2,3-dioxygenase. IDO1 is a ubiquitously expressed cytoplasmic protein with a predicted molecular weight of approximately 45 kD. It is one of the best known IFN-γ inducible genes. The product of IDO gene catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine. IDO has been implicated to protect against intracellular and extracellular pathogens. It also has been shown to maintain the special immune suppressive status of immune-privileged sites such as the brain, eyes, kidney, and placenta.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant protein, full length human IDO
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_3662230 (BioLegend Cat. No. 335853)
AB_3662230 (BioLegend Cat. No. 335854)

Antigen Details

Structure
Heme-containing enzyme of 403 amino acids with a predicted molecular weight of 45 kD.
Distribution

Cytoplasm

Function
Dioxygenase, Oxidoreductase
Ligand/Receptor
Heme, Iron, Metal binding
Cell Type
Dendritic cells, Eosinophils, Langerhans cells, Macrophages, Schwann Cells, T cells
Biology Area
Immuno-Oncology, Immunology, Inhibitory Molecules
Molecular Family
Enzymes and Regulators
Antigen References
  1. Yamazaki F, et al.1985. The Biochemical Journal. 230: 635–8.
  2. Prendergast GC, et al. 2014. Frontiers in Immunology. 5:585.
  3. Habara-Ohkubo A, et al. 1991. Gene. 105:221.
  4. Munn DH, et al. 2002. Science. 297:1867.
  5. Frumento G, et al. 2002. J Exp Med. 196:459.
  6. Muller AJ, et al. 2005. Nature Med. 11:312.
  7. Löb S, et al. 2009. Nature Rev Cancer. 9:445-52.
Gene ID
15930 View all products for this Gene ID
UniProt
View information about IDO1 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 06.11.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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