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PE anti-human LAP Recombinant Antibody

Pricing & Availability
Clone
QA20A25 (See other available formats)
Regulatory Status
RUO
Other Names
Latency Associated Peptide (LAP), Transforming growth factor beta 1 (TGF-b1), TGFB1, DPD1, TGF-beta1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
QA20A25_PE_LAP_RECOM_Antibody_012622
Human peripheral blood mononuclear cells were stimulated with PHA for 2 days. Cells were then stained with anti-human CD4 APC and anti-human LAP recombinant (clone QA20A25) PE (left) or mouse IgG1, κ PE (right).
  • QA20A25_PE_LAP_RECOM_Antibody_012622
    Human peripheral blood mononuclear cells were stimulated with PHA for 2 days. Cells were then stained with anti-human CD4 APC and anti-human LAP recombinant (clone QA20A25) PE (left) or mouse IgG1, κ PE (right).
See PE spectral data
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364403 25 tests 146 CHF
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364404 100 tests 360 CHF
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Description

TGF-β1 (transforming growth factor β1) is a multifunctional cytokine which regulates cellular proliferation and differentiation. It is ubiquitously expressed by many types of cells. Platelets express high level of TGF-β. TGF-β is synthesized as a large protein precursor and then secreted as a complex of TGF-β and LAP (latency-associated peptide), in which LAP noncovalently associates with the dimeric mature TGF-β to prevent its activity. TGF-β requires activation before it binds to its receptors and exerts functions. It has been reported that LAP-TGF-β binds to the integrins αvβ1, αvβ6, αvβ8, and α8β1 through RGD domain. TGF-β plays important roles in control proliferation and differentiation of epithelial cells, endothelial cells, fibroblasts, neurons, osteoclasts, and osteoblasts. TGF-β is believed to be important in the regulation of the development of Treg, Th17, and Th9 cells. A recent study has shown that LAP is an activated Treg surface marker.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Krammer S, et al. 2022. Cells. 12: . PubMed
RRID
AB_2910407 (BioLegend Cat. No. 364403)
AB_2910407 (BioLegend Cat. No. 364404)

Antigen Details

Structure
About 400 amino acids, N-terminal signal peptide is required for secretion from a cell, a pro-region (LAP) and C-terminal region that becomes the mature TGF-β molecule following its release from the pro-region by proteolytic cleavage.
Distribution

Ubiquitously expressed by many kinds of cells 

Function
Multifunctional cytokine, regulates cells proliferation, and differentiation
Ligand/Receptor
TGF-βRI, -RII, -RIII, CD105, LTBP, integrins
Antigen References
  1. Yi JJ, et al. 2010. Cell 142:144.
  2. Tran DQ, et al. 2009. Blood 113:5125.
  3. Lu M, et al. 2002. J. Cell. Sci. 115:4641.
  4. Khalil N. 1999. Microbes Infect. 1:1255. 
Gene ID
7040 View all products for this Gene ID
UniProt
View information about LAP on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.22.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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