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PE anti-human/mouse Galectin-9 Antibody

Pricing & Availability
Clone
W21101F (See other available formats)
Regulatory Status
RUO
Other Names
Lgals9, Gal-9, Ecalectin, Tumor antigen HOM-HD-21, Ecalectin
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
W21101F_PE_Galectin-9_Antibody_1_072324_updated
Human peripheral blood lymphocytes were stimulated with Cell Activation Cocktail (without Brefeldin A) (Cat. No. 423301) for 4 hours and surface stained with anti-human CD3 (clone UCHT1) APC. Cells were then fixed and permeabilized using the Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803), and intracellularly stained with anti-human/mouse Galectin-9 (clone W21101F) PE (left) or mouse IgG1, κ PE isotype control (right).
  • W21101F_PE_Galectin-9_Antibody_1_072324_updated
    Human peripheral blood lymphocytes were stimulated with Cell Activation Cocktail (without Brefeldin A) (Cat. No. 423301) for 4 hours and surface stained with anti-human CD3 (clone UCHT1) APC. Cells were then fixed and permeabilized using the Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803), and intracellularly stained with anti-human/mouse Galectin-9 (clone W21101F) PE (left) or mouse IgG1, κ PE isotype control (right).
  • W21101F_PE_Galectin-9_Antibody_2_071124
    MOLT-4 (human T-lymphoblastic leukemia cell line) cells were fixed and permeabilized using the Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803), then intracellularly stained with anti-human/mouse Galectin-9 (clone W21101F) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
  • W21101F_PE_Galectin-9_Antibody_3_072324_updated
    C57BL/6 mouse splenocytes were surface stained with anti-mouse CD4 (clone GK1.5) APC. Cells were then fixed and permeabilized using the Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803), then intracellularly stained with and anti-human/mouse Galectin-9 (clone W21101F) PE (left) or mouse IgG1, κ PE isotype control (right).
See PE spectral data
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390805 25 tests 175 CHF
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390806 100 tests 350 CHF
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Description

Galectin-9 is a mammalian lectin with a molecular weight around 50 kD. It is a member of the β-galactoside-binding family. With two conserved carbohydrate recognition domains (CRDs), galectin-9 binds small β-galactosides as well as complex glycoconjugates. HAVCR2/TIM3 has been reported as one of its ligands. Galectin-9 may be retained intracellularly or transported to the cell surface where it can be cleaved to generate a soluble form. Galectin-9 is expressed by lymphocytes, dendritic cells, granulocytes, eosinophils, astrocytes, endothelial cells, fibroblasts, and thymus epithelial cells. It can be induced by cytokines in various cell types and is involved in cell aggregation, adhesion, chemotaxis, and apoptosis. Galectin-9 induces regulatory T cells and suppresses Th1 and Th17 responses.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human galectin-9 protein (a.a 2-323)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
FC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our Cyto-Fast™ Fix/Perm Buffer Set. For flow cytometric staining, the suggested use of this reagent is 5 µL per 106 cells in 100 µL volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes
Clone W21101F does not block the staining of reference clone 9M1-3.
 
Recombinant protein completely blocks the staining of test clone W21101F.
RRID
AB_3662288 (BioLegend Cat. No. 390805)
AB_3662288 (BioLegend Cat. No. 390806)

Antigen Details

Structure
Member of the β-galactoside-binding lectin family. Molecular weight around 50 kD. Binds small β-galactosides as well as complex glycoconjugates with two conserved carbohydrate recognition domains (CRDs).
Distribution

Lymphocytes, dendritic cells, neutrophils, eosinophils, astrocytes, endothelial cells, fibroblasts, thymus stromal/epithelial cells

Function
Cell aggregation, adhesion, chemotaxis, apoptosis, suppression of Th1 and Th17 responses, and induction of regulatory T cells
Interaction
Astrocytes, dendritic cells, endothelial cells, eosinophils, epithelial cells, fibroblasts, lymphocytes, neutrophils, tregs
Ligand/Receptor
HAVCR2/TIM3
Cell Type
Astrocytes, Dendritic cells, Endothelial cells, Eosinophils, Epithelial cells, Fibroblasts, Lymphocytes, Neutrophils, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
Molecular Family
Adhesion Molecules, Immune Checkpoint Receptors
Antigen References
  1. Seki M, et al. 2008. Clin Immunol. 127:78-88.
  2. Tsuboi Y, et al. 2007. Clin Immunol. 124:221-33.
  3. Zhu C, et al. 2005. Nat Immunol. 6:1245-52.
  4. Dunphy JL, et al. 2002. J Biol Chem. 277:14916-24.
Gene ID
8795 View all products for this Gene ID
UniProt
View information about Galectin-9 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 07.11.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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