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PE anti-human VEGFR-3 (FLT-4) Antibody

Pricing & Availability
Clone
9D9F9 (See other available formats)
Regulatory Status
RUO
Other Names
Receptor tyrosine Kinase VEGFR-3, VEGFR3, FLT4
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
9D9F9_PE_VEGFR3_Antibody_FC_042613
Human endothelial cell line HUVEC was stained with VEGFR3 (clone 9D9F9) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
  • 9D9F9_PE_VEGFR3_Antibody_FC_042613
    Human endothelial cell line HUVEC was stained with VEGFR3 (clone 9D9F9) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
Compare all formats See PE spectral data
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356203 25 tests 153 CHF
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356204 100 tests 347 CHF
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Description

Receptor tyrosine Kinase VEGFR-3, also known as FLT4, together with VEGFR1 (FIT1) and VEGFR2 (KDR/Flk-1), are the receptors for vascular endothelial growth factors (VEGF). The VEGFR family belongs to the class II subfamily of receptor tyrosine kinases (RTKs), containing a large extracellular region which is composed of seven Ig-like domains (D1–D7), a single transmembrane, helix, and a cytoplasmic region with tyrosine kinase activity. In VEGFR-3, the fifth Ig homology domain is proteolytically cleaved which results in polypeptides that remain linked by two disulfide bonds. VEGFR-3 is widely expressed on all endothelial cells in early embryogenesis, while, in adult tissues, VEGFR-3 expression disappears from the vascular endothelial cells and is observed only on the lymphatic endothelium. VEGF-C and VEGF-D activation of VEGFR-3 plays an important role in the formation of the lymphatic vessel system. Aberrant activation or expression of VEGFR and their ligands has been implicated in tumor angiogenesis, coronary artery disease, diabetic blindness, and other diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
VEGFR extracellular domain protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for relevant formats) include: immunohistochemistry staining of frozen or paraffin embedded sections1-3.

Application References

(PubMed link indicates BioLegend citation)
  1. Jussila L, et al. 1998. Cancer Res. 58:1599. (IHC)
  2. Kilic N, et al. 2007. Blood 110:4223. (IHC)
  3. Folpe A, et al. 2000. Mod. Pathol. 13:180. (IHC)
Product Citations
  1. Connor T, et al. 2022. Methods Mol Biol. 2475:47. PubMed
  2. Tuohinto K, et al. 2023. PLoS Pathog. 19:e1010753. PubMed
  3. Lee JY, et al. 2023. Cancer Immunol Immunother. :. PubMed
  4. Lokmic Z, et al. 2015. J Vis Exp. 99: 52691. PubMed
  5. Nishino K, et al. 2021. Sci Rep. 11:8406. PubMed
  6. Nathan AA, et al. 2017. Trop Med Int Health. 22:1414. PubMed
  7. Vachon L, et al. 2022. Theranostics. 12:1440. PubMed
  8. Xu J, et al. 2017. Clin Cancer Res. 23:4482. PubMed
  9. Park S, et al. 2021. Int J Stem Cells. 14:400. PubMed
RRID
AB_2562037 (BioLegend Cat. No. 356203)
AB_2562037 (BioLegend Cat. No. 356204)

Antigen Details

Structure
Seven Ig-like domains in the extracellular domain, a single transmembrane helix, and a cytoplasmic region with tyrosine kinase activity
Distribution

Widely expressed in early embryogenesis endothelial cells, restrictly expressed on lymphatic endothelial cells in adults

Function
Mediates lymphangiogenesis in response to VEGF-C and VEGF-D, involved in cardiovascular development during embryogenesis
Ligand/Receptor
VEGF-C, VEGF-D
Biology Area
Cell Biology, Immunology, Signal Transduction
Molecular Family
Cytokine/Chemokine Receptors
Antigen References

1. Tammela T, et al. 2008. Nature 454:656.
2. Partanen TA, et al. 2000. FASEB J. 14:2087.
3. Valtola R, et al. 1999. Am. J. Pathol. 154:1381.
4. Jussila L, et al. 1998. Cancer Res. 58:1599.
5. Galland F, et al. 1992. Genomics 13:475.

Gene ID
2324 View all products for this Gene ID
UniProt
View information about VEGFR-3 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 09.22.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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