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PE anti-IDO1 Antibody

Pricing & Availability
Clone
2E2/IDO1 (See other available formats)
Regulatory Status
RUO
Other Names
Indolamine 2,3-dioxygenase, Indole 2,3-dioxygenase, Indoleamine-pyrrole 2,3-dioxygenase
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
2E2slashIDO1_PE_IDO1_Antibody_122823
Mouse IDO1 transfected 293 cells were treated overnight (left) or untreated (right) with doxycycline, then fixed & permeabilized using Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803), and intracellularly stained with anti-IDO1 (clone 2E2/IDO1) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
  • 2E2slashIDO1_PE_IDO1_Antibody_122823
    Mouse IDO1 transfected 293 cells were treated overnight (left) or untreated (right) with doxycycline, then fixed & permeabilized using Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803), and intracellularly stained with anti-IDO1 (clone 2E2/IDO1) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
654005 25 µg 176 CHF
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654006 100 µg 393 CHF
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Description

IDO1 is also known as Indolamine 2,3-dioxygenase, Indole 2,3-dioxygenase, and Indoleamine-pyrrole 2,3-dioxygenase. IDO is a ubiquitously expressed cytoplasmic protein with a predicted molecular weight of approximately 45 kD. Indoleamine 2,3-dioxygenase (IDO) is one the best known IFN-γ inducible genes. The product of IDO gene catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine. IDO has been implicated to protect against intracellular and extracellular pathogens. It also has been shown to maintain the special immune suppressive status of immune-privileged sites such as the brain, eyes, kidney, and placenta.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant mouse IDO1.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 2E2/IDO1 reacts with mouse IDO1. It does not appear to react with mouse IDO2.

Clone 2E2/IDO1 is the same clone as anti-IDO1 clone 2E2.6.

RRID
AB_3097459 (BioLegend Cat. No. 654005)
AB_3097459 (BioLegend Cat. No. 654006)

Antigen Details

Structure
403 amino acids with a predicted molecular weight of 45 kD.
Distribution

Cytoplasm

Function
Indoleamine 2,3-dioxygenase (IDO) is an IFN-γ inducible gene. It catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine. IDO has been implicated to protect against intracellular and extracellular pathogens.
Interaction
This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin.
Biology Area
Cell Biology, Immunology
Antigen References

1. Habara-Ohkubo A, et al. 1991. Gene 105:221.
2. Munn DH, et al. 2002. Science 297:1867.
3. Frumento G, et al. 2002. J. Exp. Med. 196:459.
4. Muller AJ, et al. 2005. Nature Med. 11:312.
5. Lob S, et al. 2009. Nature Rev. Cancer 9:445.

Gene ID
15930 View all products for this Gene ID
UniProt
View information about IDO1 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 12.28.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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