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PE anti-mouse CD107a (LAMP-1) Antibody

Pricing & Availability
Clone
1D4B (See other available formats)
Regulatory Status
RUO
Other Names
Lysosome-associated membrane glycoprotein 1, lysosomal membrane glycoprotein 1, CD107 antigen-like family member A, lysosome-associated membrane protein 1
Isotype
Rat IgG2a, κ
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Product Citations
publications
1D4B_PE_083109
Thioglycollate-elicited BALB/c mouse peritoneal macrophages stained with 1D4B PE
  • 1D4B_PE_083109
    Thioglycollate-elicited BALB/c mouse peritoneal macrophages stained with 1D4B PE
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Cat # Size Price Quantity Check Availability Save
121611 50 µg 122 CHF
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121612 200 µg 323 CHF
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Description

CD107a, also known as Lysosome-Associated Membrane Protein 1 (LAMP-1) or LGP-120, is a 110-140 kD type I membrane glycoprotein. Mature CD107a is heavily glycosylated from a 40 kD core protein. This molecule is located on the luminal side of lysosomes. Upon activation, CD107a is transferred to the cell membrane surface of activated platelets, activated lymphocytes, macrophages, epithelial cells, endothelial cells, and some tumor cells. CD107a has been suggested to play a role in the protection of lysosomal membrane from lysosomal hydrolases which is involved in cell adhesion and regulation of tumor metastasis, and mediates autoimmune disease progression. CD107a is a ligand for galaptin and E-selectin. Surface expression of LAMP-1 has been shown to correlate with CD8+ T cell and NK cell cytotoxicity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
This monoclonal antibody was raised against NIH/3T3 mouse embryonic fibroblast tissue culture cell membranes. It has been mapped to the N-terminus of LAMP-1.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Sun L, et al. 2021. Cancer Cell. 39:1361. PubMed
  2. Dong MB, et al. 2020. Cell. 178(5):1189-1204.e23.. PubMed
  3. Aghayev T, et al. 2022. Cancer Discov. 12:1960. PubMed
  4. Liang Y, et al. 2022. Theranostics. 12:7729. PubMed
  5. Ye L, et al. 2022. Cell Metab. 34:595. PubMed
  6. Cheng MI, et al. 2023. Nat Immunol. 24:780. PubMed
  7. Afolabi LO, et al. 2021. Front Immunol. 12:701671. PubMed
  8. Holder K, et al. 2013. J Immunol. 191:3308. PubMed
  9. Blumenthal D, et al. 2020. eLife. 9:e55995.. PubMed
  10. Lu SX, et al. 2021. Cell. . PubMed
  11. Utz B, et al. 2020. Sci Rep. 10:18733. PubMed
  12. Adams NM et al. 2018. Immunity. 48(6):1172-1182 . PubMed
  13. Wiedemann GM, et al. 2020. Cell Rep. 33:108498. PubMed
  14. Niemann J, et al. 2019. Nat Commun. 10:3236. PubMed
  15. Diaz–Salazar C, et al. 2020. Cell Rep. 32:108186. PubMed
  16. Peauroi EM, et al. 2022. iScience. 25:105510. PubMed
  17. Vicencio JM, et al. 2022. Cell Death Dis. 13:274. PubMed
  18. Zhu M, et al. 2015. J Immunol. 195: 4492 - 4502. PubMed
  19. Rio M, et al. 2012. J Immunol. 188:4885. PubMed
  20. Yang E, et al. 2016. J Immunol. 197: 934 - 941. PubMed
  21. Hekim C, et al. 2017. Cancer Immunol Res. 5:157. PubMed
  22. Frazer GL, et al. 2021. J Cell Biol. 220:. PubMed
  23. Chitirala P, et al. 2020. eLife. 9:00. PubMed
  24. Matsumoto M, et al. 2021. Cell Host Microbe. 29(6):930-940.e4. PubMed
  25. Sheppard S, et al. 2021. Cell Reports. 35(9):109210. PubMed
  26. Meng Michelle Xu et al. 2017. Immunity. 47(2):363-373 . PubMed
  27. DiFranco K, et al. 2012. J Biol Chem. 287:17618. PubMed
  28. Geary CD et al. 2018. Cell reports. 24(8):1949-1957 . PubMed
  29. Liu CF, et al. 2017. Front Immunol. 1.445833333. PubMed
  30. Li X, et al. 2021. Front Cell Dev Biol. 9:647713. PubMed
RRID
AB_1732051 (BioLegend Cat. No. 121611)
AB_1732051 (BioLegend Cat. No. 121612)

Antigen Details

Structure
LAMP-1 is a 406 amino acid protein with a molecular mass of 44 kD.
Gene ID
16783 View all products for this Gene ID
UniProt
View information about CD107a on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 10.22.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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