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PE anti-mouse CD115 (CSF-1R) Antibody

Pricing & Availability
Clone
AFS98 (See other available formats)
Regulatory Status
RUO
Other Names
CSF-1R, M-CSFR, c-fms
Isotype
Rat IgG2a, κ
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Product Citations
publications
A_AFS98_PE_102309
Thioglycolate-elicited BALB/c mouse peritoneal macrophages stained with rat IgG1, k PE and CD11b FITC.
  • A_AFS98_PE_102309
    Thioglycolate-elicited BALB/c mouse peritoneal macrophages stained with rat IgG1, k PE and CD11b FITC.
  • B_AFS98_PE_102309
    Thioglycolate-elicited BALB/c mouse peritoneal macrophages stained with AFS98 PE and CD11b FITC.
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135505 50 µg 117 CHF
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135506 200 µg 360 CHF
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Description

CSF-1R, also known as CD115 and M-CSFR, is a single-pass type I membrane protein and member of the platelet-derived growth factor receptor family. This c-fms (Fms proto-oncogene) gene product's natural ligands include M-CSF and IL-34. Structural studies of CD115 have described an Ig-like extracellular domain, a transmembrane domain, an intracellular juxtamembrane domain, a split tyrosine kinase domain, and a C-terminal tail receptor. Receptor activation induces homodimerization in addition to phosphorylation and ubiquitination of intracellular residues. CD115 directly influences tissue macrophage and osteoclast differentiation and proliferation. It is expressed on monocytes/macrophages, peritoneal exudate cells, plasmacytoid and conventional dendritic cells, and osteoclasts.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking of ligand binding1. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays.

It has been reported that CD115 can be rapidly internalized, especially when samples are exposed to room temperature. Approximate 33% decrease in CD115 expression has been observed between 0 and 4 hours after sample collection, while overnight incubation of the cells results in complete CD115 downregulation. Pre-treatment with EDTA and low temperatures (2 to 8°C) helps in maintaining surface expression of CD1154.

Application References

(PubMed link indicates BioLegend citation)
  1. Sudo T, et al. 1995. Oncogene 11:2469.
  2. Murayama T, et al. 1999. Circulation 99:1740.
  3. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. PubMed
  4. Breslin WL, et al. 2013. J Immunol Methods. 390(1-2):1 PubMed
  5. Dong L, et al. 2016. Nature. 539:304–308. PubMed
Product Citations
  1. Combes F, et al. 2018. Neoplasia. 20:848. PubMed
  2. Sierro F, et al. 2017. Immunity. 47:374. PubMed
  3. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  4. Rosina M, et al. 2022. Cell Metab. 34:533. PubMed
  5. Liang Y, et al. 2021. Cell Death Differ. 28:2728. PubMed
  6. Cohen K, et al. 2021. Cell Rep. 37:110026. PubMed
  7. Li T, et al. 2022. J Exp Med. 219: . PubMed
  8. Dolfi B, et al. 2022. Cell Rep. 39:110949. PubMed
  9. Roberts JL, et al. 2022. Bone. 157:116310. PubMed
  10. Fernández-García V, et al. 2022. Biomed Pharmacother. 148:112769. PubMed
  11. Abdel-Haq R, et al. 2022. Elife. 11:. PubMed
  12. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  13. Suresh R, et al. 2020. J Immunother Cancer. 8:. PubMed
  14. Groenen AG, et al. 2022. J Lipid Res. 63:100167. PubMed
  15. Tripathi H, et al. 2020. J Mol Cell Cardiol. 149:95. PubMed
  16. Bouchareychas L, et al. 2021. iScience. 24(8):102847. PubMed
  17. Oliveira-Lima OC, et al. 2015. Immunobiology. . PubMed
  18. Jackson A, et al. 2014. J Leukoc Biol. 92:609. PubMed
  19. Takacs E, et al. 2017. J Immunol. 198:239. PubMed
  20. Lee MH, et al. 2018. J Immunol Res. 2018:1601079. PubMed
  21. Tan X, et al. 2016. PLoS One. 11: 0160985. PubMed
  22. Johnson KD, et al. 2022. Blood Adv. 6:1464. PubMed
  23. Bratti M, et al. 2022. Front Immunol. 13:1029759. PubMed
  24. Hreha TN, et al. 2020. Front Immunol. 1.597916667. PubMed
  25. Sakai M, et al. 2020. Immunity. 51(4):655-670. PubMed
  26. Garcia–Agudo LF, et al. 2019. FASEB J. :fj201900337R. PubMed
  27. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  28. Fuster JJ, et al. 2020. Cell Rep. 33:108326. PubMed
  29. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  30. LeBlond ND, et al. 2020. J Lipid Res. 61:1697. PubMed
  31. Yang F, et al. 2020. Cancer Res. 80:3677. PubMed
  32. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  33. Smith C, et al. 2012. Cancer Discovery. 2:722. PubMed
  34. Distel E, et al. 2014. Circ Res . 115:759. PubMed
  35. Voisin M, et al. 2021. Commun Biol. 4:420. PubMed
  36. Eisele AS, et al. 2022. Elife. 11:. PubMed
  37. Barman PK, et al. 2019. J Immunol. 202:2720. PubMed
  38. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  39. Anderson SR, et al. 2019. Cell Rep. 27:2002. PubMed
  40. La Rose AM, et al. 2021. Mol Metab. 53:101265. PubMed
  41. Tripathi H, et al. 2020. Stem Cell Rev Rep. 0.953472222. PubMed
  42. Kuhn JA, et al. 2021. Elife. 10:. PubMed
  43. Aktories P, et al. 2022. Cell Rep Methods. 2:100260. PubMed
  44. Ding P, et al. 2022. Bone Res. 10:42. PubMed
  45. Bouchareychas L, et al. 2020. Cell Reports. 32(2):107881. PubMed
  46. Gadd V, et al. 2016. PLoS One. 11: 0157771. PubMed
  47. Li W, et al. 2021. PLoS Pathog. 17:e1009462. PubMed
  48. Wang W, et al. 2021. J Am Heart Assoc. 10:e019142. PubMed
  49. Johansson J, et al. 2013. J Neurosci. 33:16016. PubMed
  50. Burgener SS, et al. 2019. Cell Rep. 27:3646. PubMed
  51. Clemente C, et al. 2018. Nat Commun. 9:910. PubMed
  52. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  53. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  54. LeBlond ND, et al. 2020. J Lipid Res. 61:1697. PubMed
  55. Ulrich V, et al. 2016. EMBO Mol Med. 8: 643 - 653. PubMed
  56. Mairhofer D, et al. 2015. J Invest Dermatol. 135: 2785-93. PubMed
  57. Chen S, et al. 2014. Biochem Biophys Res Commun. 446:1002. PubMed
  58. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  59. Foster G, et al. 2015. J Immunol. 195: 5380 - 5392. PubMed
  60. Aryal B, et al. 2016. Nat Commun. 7:12313. PubMed
  61. Ryu J, et al. 2013. Circulation. 127:710. PubMed
  62. Jain A, et al. 2020. Nat Immunol. 0.920138889. PubMed
  63. Petriello MC, et al. 2018. Toxicol Sci. 162:548. PubMed
  64. Hussein M, et al. 2015. PLoS One. 10: 0135218. PubMed
  65. Tran S, et al. 2020. Immunity. 53(3):627-640.e5. PubMed
  66. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  67. Steinmetz O, et al. 2016. J Infect Dis. 10.1093/infdis/jiv763. PubMed
  68. Yáñez A et al. 2017. Immunity. 47(5):890-902 . PubMed
  69. Di Liberto G et al. 2018. Cell. 175(2):458-471 . PubMed
RRID
AB_1937253 (BioLegend Cat. No. 135505)
AB_1937253 (BioLegend Cat. No. 135506)

Antigen Details

Structure
Ig superfamily, 145 kD
Distribution

Monocytes/macrophages, peritoneal exudate cells, plasmacytoid and conventional dendritic cells, osteoclasts

Function
Growth factor receptor, tyrosine kinase
Ligand/Receptor
Macrophage colony stimulating factor (M-CSF), IL-34
Cell Type
Dendritic cells, Macrophages, Monocytes, Osteoclasts
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References
  1. Sudo T, et al. 1995 Oncogene 11:2469.
  2. Murayama T, et al. 1999 Circulation 99:1740.
  3. Goswami S, et al. 2005 Cancer Res. 65:5278.
  4. Yu W, et al. 2008 J. Leuko. Biol. 84:852.
Gene ID
12978 View all products for this Gene ID
UniProt
View information about CD115 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Why do I have a weak CD115 staining?

It has been reported that CD115 can be rapidly internalized, especially when samples are exposed to room temperature. Approximate 33% decrease in CD115 expression has been observed between 0 and 4 hours after sample collection, while overnight incubation of the cells results in complete CD115 downregulation.  Pre-treatment with EDTA and low temperatures (2 - 8°C) helps in maintaining surface expression of CD115. In addition, brief fixation of the cells with Fixation Buffer (Cat. No. 420801) for 10 minutes will block CD115 internalization.

Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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