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PE anti-mouse CD122 (IL-2Rβ) Antibody

Pricing & Availability
Clone
TM-β1 (See other available formats)
Regulatory Status
RUO
Other Names
IL-2 Receptor β chain, IL-2Rβ
Isotype
Rat IgG2b, κ
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Product Citations
publications
TM-beta1_PE_021808
C57BL/6 mouse splenocytes stained with TM-β1 PE
  • TM-beta1_PE_021808
    C57BL/6 mouse splenocytes stained with TM-β1 PE
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Cat # Size Price Quantity Check Availability Save
123209 25 µg 86 CHF
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123210 100 µg 214 CHF
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Description

CD122 is a 70-75 kD IL-2 receptor β chain also known as IL-2Rβ, which is also shared by the IL-15 receptor. It is constitutively expressed by NK cells and at lower levels by T cells, B cells, monocytes, and macrophages. The IL-2Rβ chain can combine with either the common γ subunit (γc, CD132) alone or with the γc subunit and the IL-2Rα subunit (CD25) to generate intermediate or high affinity IL-2 receptor complexes, respectively. CD122 expression levels can be upregulated by activation. The TM-β1 antibody does inhibit IL-2 binding to the IL-2 receptor. CD122 is expressed on murine, but not human, CD8+ Tregs involved in the maintenance of T cell homeostasis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Rat T cell line expressing mouse CD122 ( IL-2Rβ)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, blocking of IL-2 binding1, and NK cell depletion2 in vivo. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for blocking of IL-2 binding in vivo and in vitro (Cat. No. 123204).

Application References

(PubMed link indicates BioLegend citation)
  1. Tanaka T, et al. 1991. J. Immunol. 147:2222.
  2. Tanaka T, et al. 1993. J. Exp. Med. 178:1103.
  3. Tanaka T, et al. 1992. Int. Immunol. 4:487.
Product Citations
  1. Li DD, et al. 2022. J Cell Mol Med. 26:2438. PubMed
  2. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
  3. Pokrovskii M, et al. 2020. EMBO J. 39:e104159. PubMed
  4. Reyes RM, et al. 2021. Oncoimmunology. 10:2006529. PubMed
  5. Sekiya T et al. 2018. Cell reports. 24(6):1627-1638 . PubMed
  6. Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
  7. De Simone G, et al. 2021. Immunity. :. PubMed
  8. Tomala J, et al. 2020. Methods Mol Biol. 2111:101. PubMed
  9. Jandke A, et al. 2020. Nat Commun. 3.075694444. PubMed
  10. Yue X, et al. 2021. EMBO Rep. 22:e52716. PubMed
  11. Fan MY et al. 2018. Cell reports. 25(5):1204-1213 . PubMed
  12. Hu Z, et al. 2013. J Immunol. 191:312. PubMed
  13. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  14. Long L, et al. 2021. Nature. 600:308. PubMed
  15. Wu C, et al. 2018. Scand J Immunol. 87:e12661. PubMed
RRID
AB_940617 (BioLegend Cat. No. 123209)
AB_940617 (BioLegend Cat. No. 123210)

Antigen Details

Structure
Ig superfamily, forms high affinity IL-2 receptor with CD25 and CD132 chains or intermediate affinity receptor with CD132 alone, 70-75 kD
Distribution
T and B cells, NK cells, monocytes, macrophages
Function
Critical component of IL-2 and IL-15 signaling
Ligand/Receptor
IL-2, IL-15
Cell Type
B cells, Macrophages, Monocytes, NK cells, T cells, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Minami Y, et al. 1993. Annu. Rev. Immunol. 11:245.
3. Suzuki H, et al. 1995. Science 268:1472.
4. Shi Z, et al. 2009. Eur. J. Immunol. 39:2109.

Gene ID
16185 View all products for this Gene ID
UniProt
View information about CD122 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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