- Clone
- VECD1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Vascular endothelial-cadherin, VE-cadherin
- Isotype
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
138105 | 25 µg | 134 CHF | ||||
138106 | 100 µg | 365 CHF |
CD144, also known as vascular endothelial-cadherin (VE-cadherin), is a 120 kD member of the type II Cadherin family. It is an endothelial specific hemophilic adhesion molecule involved in endothelial cell survival, migration, contact-dependent growth inhibition and homophilic adhesion. VE-cadherin is essential for maintaining the integrity of the endothelial barrier in vivo.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- VE-cadherin protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
It is also recommended using EDTA-based solutions for dissociating attachment-dependent cell lines. - Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Additional reported applications (for relevant formats) include: Immunoprecipitation1, Western Blot1, functional assay2, 3 by inhibiting vascular endothelial cell-cell interactions and immunocytochemistry staining of cultured cell lines4.
-
Application References
(PubMed link indicates BioLegend citation) -
- Allport JR, et al. 2002. J. Leukoc. Biol. 71:821. (IP WB)
- Hirashima M, et al. 2009. Blood 93:1253. (Block)
- Matsuyoshi N, et al. 1997. Proc. Assoc. Am. Physicians 109:362. (Block)
- Matsumura K, et al. 2003. Blood 101:1367. (IF)
- Hirashima M, et al. 2009. Blood 101:2261. (FC)
- Tanaka Y, et al. 2012. PNAS. 109:4515. PubMed.
- Product Citations
-
- RRID
-
AB_2077941 (BioLegend Cat. No. 138105)
AB_2077941 (BioLegend Cat. No. 138106)
Antigen Details
- Structure
- A 120 kD member of type II Cadherin family
- Distribution
-
Endothelial cells
- Function
- Involved in endothelial cell survival, migration, contact-dependent growth inhibition, and hemophilic adhesion.
- Cell Type
- Endothelial cells, Mesenchymal Stem Cells
- Biology Area
- Immunology, Innate Immunity, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Gotsch U, et al. 1997. J. Cell Sci. 110:583.
2. Kataoka H, et al. 1997. Dev. Growth Differ. 39:729. - Gene ID
- 12562 View all products for this Gene ID
- UniProt
- View information about CD144 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All CD144 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD144 (VE-cadherin) | VECD1 | FC,IP,WB,ICC |
PE anti-mouse CD144 (VE-cadherin) | VECD1 | FC |
Alexa Fluor® 647 anti-mouse CD144 (VE-cadherin) | VECD1 | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-mouse CD144 (VE-cadherin)
Mouse endothelial cell b.End.3 stained with purified VECD1 c... -
PE anti-mouse CD144 (VE-cadherin)
Mouse endothelial cell b.End.3 stained with VECD1 PE -
Alexa Fluor® 647 anti-mouse CD144 (VE-cadherin)
Mouse endothelial cell bEnd.3 stained with VECD1 Alexa Fluor...
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