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PE anti-mouse CD150 (SLAM) Antibody

Pricing & Availability
Clone
TC15-12F12.2 (See other available formats)
Regulatory Status
RUO
Other Names
Signaling Lymphocyte Activation Molecule (SLAM), IPO-3
Isotype
Rat IgG2a, λ
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Product Citations
publications
TC15-12F12.2
C57BL/6 mouse splenocytes were stained with CD150 (clone TC15-12F12.2) PE (solid line) or rat IgG2a PE isotype control (broken line).
  • TC15-12F12.2
    C57BL/6 mouse splenocytes were stained with CD150 (clone TC15-12F12.2) PE (solid line) or rat IgG2a PE isotype control (broken line).
  • TC15-12F12_PE_2_102110
    C57BL/6 mouse bone marrow cells were stained with CD150 (clone TC15-12F12.2) PE (filled histogram) or rat IgG2a PE isotype control (open histogram) (gated on lymphoid cell population).
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Cat # Size Price Quantity Check Availability Save
115903 25 µg 104 CHF
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115904 100 µg 268 CHF
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Description

CD150 is a 75-95 kD member of the immunoglobulin superfamily, also known as SLAM (signaling lymphocyte activation molecule) or IPO-3. CD150, a single chain type I transmembrane molecule, is expressed on thymocytes, T cell subsets, B cells, dendritic cells, and endothelial cells. The expression is upregulated upon activation. CD150 expression has been shown to be maintained on Th1 but not Th2 clones. T regulatory cells express a relatively high level of CD150. Antibodies against CD150 have been shown to augment IFN-γ production by Th1 cells, especially when co-stimulated through the TCR. CD150 associates with the src homology 2-domain-containing protein tyrosine phosphatase SHP-2, and this association is thought to be involved in signal transduction. In combination with CD48, CD150 is a useful marker for hematopoietic stem cell studies.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse SLAM-human IgG1 fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD150 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The TC15-12F12.2 antibody has been reported to enhance the production of IFN-? by Th1 cells stimulated through TCR. Additional reported applications (for the relevant formats) include: immunoprecipitaion17, enhancing IFN-? production by Th1 cells when stimulated with CD31, and inhibiting CD3 induced T cell proliferation6. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 115949 & 115950).

Application References

(PubMed link indicates BioLegend citation)
  1. Castro AG, et al. 1999. J. Immunol. 163:5860. (FC, Costim, IP)
  2. Forsberg EC, et al. 2005. PLoS Genet. 1:e28. (FC)
  3. Terrazas LI, et al. 2005. Int. J. Parasitol. 35:1349. (FC)
  4. Cannons JL, et al. 2006. J. Exp. Med. 203:1551. (FC)
  5. Umemoto T, et al. 2006. J. Immunol. 177:7733. (FC)
  6. Jordan MA, et al. 2007. J. Immunol. 178:1618. (FC, Block) PubMed
  7. Jung Y, et al. 2007. Blood 110:82. PubMed
  8. Pimanda JE, et al. 2007. Proc. Natl. Acad. Sci. USA 104:840.
  9. Sugiyama T, et al. 2007. Proc. Natl. Acad. Sci. USA 104:175.
  10. Kim I, et al. 2006. Blood 108:737. PubMed
  11. Ema H, et al. 2006. Nat Protoc. 1:2979. PubMed
  12. Fraser ST, et al. 2007. Blood 109:4616. PubMed
  13. Jung Y, et al. 2008. Stem Cells. 26:2042. Pubmed
  14. Song J, et al. 2010. Blood 115:2592. PubMed
  15. Cridland SO, et al. 2009. Blood Cell. Mol. Dis. 43:149. (FC) PubMed
  16. Morita Y, et al. 2010. J. Exp Med. 207:1173. PubMed
  17. Talaei N, et al. 2015. J. Immunol. 195(10):4623. PubMed
Product Citations
  1. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
  2. Ho TT, et al. 2021. J Exp Med. 218:. PubMed
  3. Zhang D, et al. 2022. Cell Stem Cell. 29:232. PubMed
  4. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  5. Tátrai P, et al. 2022. EMBO J. 41:e108739. PubMed
  6. Xu Y, et al. 2023. EMBO Rep. 24:e55503. PubMed
  7. Huang D, et al. 2023. Exp Hematol Oncol. 12:11. PubMed
  8. Yuan O, et al. 2022. Sci Adv. 8:eabm9987. PubMed
  9. Meacham CE, et al. 2022. Nat Cell Biol. 24:697. PubMed
  10. Jabbar S, et al. 2022. Exp Hematol Oncol. 11:83. PubMed
  11. Hu W, et al. 2015. Blood. 125:2206. PubMed
  12. Pietras E, et al. 2016. Nat Cell Biol. 10.1038/ncb3346. PubMed
  13. Chen Q, et al. 2019. Cell Stem Cell. 25(6):768-783.e6. PubMed
  14. Soukup AA, et al. 2019. J Clin Invest. 129:1180. PubMed
  15. Shiroshita K, et al. 2022. Cell Rep Methods. 2:100354. PubMed
  16. Kang YA, et al. 2023. J Exp Med. 220:. PubMed
  17. Meng Y, et al. 2023. Cell Mol Biol Lett. 28:45. PubMed
  18. Nita A, et al. 2021. Cell Reports. 34(5):108688. PubMed
  19. Maltby S, et al. 2014. J Immunol. 193:4072. PubMed
  20. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  21. Thomas GE, et al. 2022. Nat Cell Biol. 24:872. PubMed
  22. Yang X, et al. 2020. Blood Sci. 2:89. PubMed
  23. Wei Q, et al. 2021. Nat Commun. 12:2522. PubMed
  24. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  25. Fujino T, et al. 2021. Nat Commun. 12:1826. PubMed
  26. Li CC, et al. 2022. Nat Commun. 13:346. PubMed
  27. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  28. Yamamoto K, et al. 2021. Cell Reports. 36(8):109576. PubMed
  29. Gao B, et al. 2018. Aging Cell. 3. PubMed
  30. Zhao M, et al. 2019. Cell Rep. 26:652. PubMed
  31. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  32. Kobayashi H, et al. 2020. STAR Protoc. 1:100078. PubMed
  33. Jung Y, et al. 2008. Stem Cells. 26:2042. PubMed
  34. Kokkaliaris K, et al. 2016. Blood. 128: 1181 - 1192. PubMed
  35. Greenbaum A, et al. 2012. Blood. 120:295. PubMed
  36. Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed
  37. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  38. Kim SP, et al. 2021. Cell Reports. 36(9):109626. PubMed
  39. Ganuza M, et al. 2017. Exp Hematol. 10.1016/j.exphem.2017.04.006. PubMed
  40. Stivala S, et al. 2019. J Clin Invest. 130:1596. PubMed
  41. Baccelli I, et al. 2020. Cancer Cell. 36(1):84-99. PubMed
  42. Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed
  43. Kinkel SA, et al. 2022. iScience. 25:104684. PubMed
  44. Jia W, et al. 2021. Nat Commun. 12:2118. PubMed
  45. Leiva M, et al. 2016. Nat Commun. 7:10222. PubMed
  46. Grassinger J, et al. 2012. Cytokine. 58:218. PubMed
  47. Jung Y, et al. 2011. Exp Hematol. 39:151. PubMed
  48. Jordan M, et al. 2007. J Immunol. 178:1618. PubMed
  49. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  50. Mooney C, et al. 2017. International Journal of Molecular Sciences. 10.3390/ijms18051037. PubMed
  51. Dong S, et al. 2021. Nature. 591:117. PubMed
  52. Zhang J, et al. 2015. Leukemia. 29 1847 . PubMed
  53. Bahal R, et al. 2016. Nat Commun. 7:13304. PubMed
  54. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
  55. Breitbach M, et al. 2018. Cell Stem Cell. 1.098611111. PubMed
  56. Tadokoro Y, et al. 2018. Cell Stem Cell. 1.411805556. PubMed
  57. Ise W, et al. 2018. Immunity. 48:702. PubMed
  58. Comazzetto S et al. 2018. Cell stem cell. 24(3):477-486 . PubMed
  59. Kim I, et al. 2006. Blood. 108:737. PubMed
  60. Pinho S, et al. 2022. Nat Cell Biol. 24:290. PubMed
  61. Ye P, et al. 2022. Front Cardiovasc Med. 8:810477. PubMed
  62. Witkowski MT, et al. 2020. Cancer Cell. 37:867. PubMed
  63. Montecino-Rodriguez E, et al. 2020. STAR Protoc. 1:100159. PubMed
  64. Chiba H, et al. 2013. Am J Physiol Cell Physiol. 305:693. PubMed
  65. Morita Y, et al. 2010. J Exp Med. 207:1173. PubMed
  66. Jung Y, et al. 2007. Blood . 110:82. PubMed
  67. Nagai M, et al. 2020. Cell. 178(5):1072-1087.e14.. PubMed
  68. Hsu JI et al. 2018. Cell stem cell. 23(5):700-713 . PubMed
  69. Reismann D, et al. 2017. Nat Commun.. 10.1038/s41467-017-01538-9. PubMed
  70. Du J, et al. 2012. Stem Cells. 30:1447. PubMed
  71. Nosho S, et al. 2020. Int J Mol Sci. 21:00. PubMed
  72. Kruta M, et al. 2021. Cell Stem Cell. :. PubMed
  73. Seneviratne AK, et al. 2019. Cell Stem Cell. 24:621. PubMed
  74. Nacson J, et al. 2020. Molecular Cell. 78(5):951-959. PubMed
  75. Yang S, et al. 2022. J Exp Med. 219:. PubMed
  76. Sacma M, et al. 2022. STAR Protoc. 3:101483. PubMed
  77. Yi W, et al. 2021. Cell Reports. 34(13):108922. PubMed
  78. Song J, et al. 2010. Blood. 115:2592. PubMed
  79. van Oostrum M, et al. 2019. Nat Commun. 4.398611111. PubMed
  80. Williams JW, et al. 2017. Circ Res. 121:662. PubMed
  81. Pierce H, et al. 2017. Cell Stem Cell. 1.283333333. PubMed
  82. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  83. Pinho S et al. 2018. Developmental cell. 44(5):634-641 . PubMed
  84. Xia P et al. 2018. Immunity. 48(4):688-701 . PubMed
  85. Thongthip S, et al. 2016. Genes Dev. 30: 645 - 659. PubMed
  86. Philip E Boulais et al. 2018. Immunity. 49(4):627-639 . PubMed
  87. Balzano M et al. 2019. Cell reports. 26(12):3257-3271 . PubMed
  88. Kobayashi H, et al. 2020. Cell Reports. 28(1):145-158.e9.. PubMed
  89. Garaycoechea JI, et al. 2018. Nature. 553:171. PubMed
  90. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  91. Zhang CR, et al. 2022. Blood Cancer Discov. 3:220. PubMed
  92. Wei Q, et al. 2020. Dev Cell. 53:503. PubMed
  93. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  94. Wang X, et al. 2018. Stem Cell Reports. 11:274. PubMed
  95. Hoffmann J, et al. 2021. Nat Commun. 12:3964. PubMed
  96. Fischer KC, et al. 2021. Cell Death Dis. 12:28. PubMed
  97. Omatsu Y, et al. 2022. Nat Commun. 13:2654. PubMed
  98. Zhang J, et al. 2015. J Biol Chem. 290 19093 . PubMed
  99. Ley C, et al. 2015. Cancer Cell. 27: 631-43. PubMed
  100. Keerthivasan S, et al. 2021. Immunity. 54(7):1511-1526.e8. PubMed
  101. Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
  102. Kaplan I, et al. 2011. J Immunol. 186:2826. PubMed
  103. Soukup AA, et al. 2021. Sci Adv. 7:eabk3521. PubMed
  104. Gao X, et al. 2021. Nature. 589:591. PubMed
  105. Alvarez S, et al. 2015. Nat Commun. 6: 8548. PubMed
  106. Theocharides A, et al. 2012. J Exp Med. 209:1883. PubMed
  107. He M, et al. 2020. Cell Metabolism. 31(3):580-591. PubMed
  108. Xu C, et al. 2018. Nat Commun. 9:2449. PubMed
  109. Magee JA, et al. 2021. Stem Cell Reports. 16:20. PubMed
  110. DeVilbiss AW, et al. 2021. eLife. 10:00. PubMed
  111. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  112. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  113. Riedel SS, et al. 2021. Molecular Cell. 81(11):2332-2348.e9. PubMed
  114. Matsuzaki Y, et al. 2015. Biomed Rep. 1: 91 - 97. PubMed
  115. Woods B, et al. 2019. Clin Cancer Res. 25:5901. PubMed
  116. Li K, et al. 2020. Nat Commun. 0.795138889. PubMed
  117. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
  118. Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed
  119. Säwen P et al. 2018. eLife. 7 pii: e41258. PubMed
  120. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  121. Yang L, et al. 2021. Front Immunol. 12:722273. PubMed
  122. Asada N, et al. 2017. Nat Cell Biol. 19:214-223. PubMed
  123. Kang YA, et al. 2020. J Exp Med. 217:00:00. PubMed
  124. Xie M, et al. 2020. Haematologica. . PubMed
  125. Zhou J, et al. 2019. Cell Stem Cell. 24:285. PubMed
  126. Fraser S, et al. 2007. Blood. 109:343. PubMed
  127. Man N, et al. 2021. JCI Insight. 6:. PubMed
  128. Gu Z, et al. 2021. Nat Genet. 53:672. PubMed
  129. Holzapfel B, et al. 2015. Biomaterials. 61: 103-114. PubMed
  130. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
RRID
AB_313682 (BioLegend Cat. No. 115903)
AB_313682 (BioLegend Cat. No. 115904)

Antigen Details

Structure
Ig superfamily, 75-95 kD
Distribution

Thymocytes, T cell subset, B lymphocytes, dendritic cells, endothelial cells

Function
B cell and dendritic cell costimulation
Ligand/Receptor
CD150
Cell Type
B cells, Dendritic cells, Endothelial cells, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Cocks BG, et al. 1995. Nature 376:260.
2. Punnonen J, et al. 1997. J. Exp. Med. 185:993.
3. Sidorenko SP, et al. 1993. J. Immunol. 151:4614.

Gene ID
27218 View all products for this Gene ID
UniProt
View information about CD150 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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