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PE anti-mouse CD16/32 Antibody

Pricing & Availability
Clone
93 (See other available formats)
Regulatory Status
RUO
Other Names
Fcγ R III/II, Ly-17
Isotype
Rat IgG2a, λ
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Product Citations
publications
93_PE_051906
C57BL/6 mouse splenocytes stained with 93 PE
  • 93_PE_051906
    C57BL/6 mouse splenocytes stained with 93 PE
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101307 50 µg 98 CHF
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101308 200 µg 304 CHF
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Description

CD16 is low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Sorted pre-B cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone 93 can be used for blocking of CD16/CD32 interactions with the Fc domain of immunoglobulins, but is not the same clone as 2.4G2.

The 93 mAb is specific to the common epitope of CD16/CD32. Additional reported applications (for the relevant formats) include: immunoprecipitation1 and blocking of Fc-mediated reactions in functional studies2,4,23. It is useful for blocking non-specific binding of immunoglobulin to Fc receptors. For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with purified anti-CD16/CD32 antibody (=1.0 µg per 106 cells in 100 µL volume) for 5-10 minutes on ice prior to immunostaining. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 101330) (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered).

Application References

(PubMed link indicates BioLegend citation)
  1. Personal communication (IP)
  2. Oliver AM, et al. 1999. Hybridoma 18:113. (Block)
  3. Brummel R and Lenert P. 2005. J. Immunol. 174:2429.
  4. Terrazas LI, et al. 2005. Int. J. Parasitol. 35:1349. (Block)
  5. Clements JL, et al. 2006. J. Immunol. 177:905.
  6. Mohamed W, et al. 2010. Infect Immun. 78:3306. PubMed
  7. Ouchi T, et al. 2011. J. Exp Med. 208:2607. PubMed
  8. Kmieciak M, et al. 2011. J. Vis. Exp. 47:2381. PubMed
  9. Yamazaki S, et al. 2012. PLoS One. 7:e51665. PubMed
  10. Li J, et al. 2012. Arthritis Rheum. 64:1098. PubMed
  11. Azuma M, et al. 2012. Oncoimmunology. 1:581. PubMed
  12. Koon HW, et al. 2013. J. Vis. Exp. 68:4208. PubMed
  13. Hegde VL, et al. 2013. J Biol Chem. 288:36810. PubMed
  14. Huang J, et al. 2013. J. Immunol Methods. 387:254. PubMed
  15. Dutow P, et al. 2014. J Infect Dis. PubMed
  16. Fan Y, et al. 2014. J Exp Med. 211:313. PubMed
  17. Huang HN, et al. 2014. Antimicrob Agents Chemother. 58:1538. PubMed
  18. Takei S, et al. 2014. Vaccine. 32:3066. PubMed
  19. Richardson ML, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed
  20. Cekanaviciute E, et al. 2014. J Immunol. 193:139. PubMed
  21. Kimura T, et al. 2014. Int Immunol. 26:697. PubMed
  22. Everad A, et al. 2014. Nat Commun. 5:5648. PubMed
  23. Cenci E, et al. 2006. J. Leuko. Biol. 79(1):40-5. (Block)
Product Citations
  1. Pannunzio B, et al. 2022. Cells. 11: . PubMed
  2. Gupta S, et al. 2022. Sci Adv. 8:eabn4886. PubMed
  3. Ye B, et al. 2022. Cell Mol Immunol. 19:619. PubMed
  4. Shiroshita K, et al. 2022. Cell Rep Methods. 2:100354. PubMed
  5. Tripathi H, et al. 2020. J Mol Cell Cardiol. 149:95. PubMed
  6. Davis FM, et al. 2020. J Immunol. 2503:204. PubMed
  7. Domingues AF, et al. 2020. Elife. 9:e51754. PubMed
  8. Li DY, et al. 2018. Sci Rep. 8:17979. PubMed
  9. Hanhai Z, et al. 2021. Aging (Albany NY). 13:13108. PubMed
  10. Chen L, et al. 2022. J Nanobiotechnology. 20:210. PubMed
  11. Xiao S, et al. 2008. J Immunol. 180:914. PubMed
  12. Sun Y et al. 2018. Cancer cell. 34(4):643-658 . PubMed
  13. Yamamoto R et al. 2018. Cell stem cell. 22(4):600-607 . PubMed
  14. Papafragkos I, et al. 2022. Front Immunol. 13:889075. PubMed
  15. Swidergall M, et al. 2019. Cell Rep. 28:423. PubMed
  16. Davis FM, et al. 2019. Arterioscler Thromb Vasc Biol. 39:2353. PubMed
  17. Xu X, et al. 2021. J Nanobiotechnology. 19:376. PubMed
  18. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  19. He C, et al. 2021. Oncoimmunology. 1897295:10. PubMed
  20. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
  21. Amo-Aparicio J, et al. 2021. Theranostics. 11:9805. PubMed
  22. Yang SH, et al. 2017. Front Immunol. 8:1192. PubMed
  23. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  24. Mooney C, et al. 2017. International Journal of Molecular Sciences. 10.3390/ijms18051037. PubMed
  25. Dong S, et al. 2021. Nature. 591:117. PubMed
  26. Omori S, et al. 2021. Cell Reports. 34(6):108734. PubMed
  27. Martìnez-Muriana A, et al. 2016. Sci Rep. 6: 25663. PubMed
  28. Zaro BW, et al. 2020. eLife. 9:00. PubMed
  29. Murakami K, et al. 2021. Cell Reports. 34(1):108579. PubMed
  30. Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed
  31. Koelwyn GJ, et al. 2020. Nat Med. 1452:26. PubMed
  32. Yokomizo-Nakano T, et al. 2020. Cancer Res. 80:2523. PubMed
  33. van der Poel CE, et al. 2019. Cell Rep. 29:2745. PubMed
  34. Rai S, et al. 2022. Nat Commun. 13:5346. PubMed
  35. Golan K et al. 2018. Cell stem cell. 23(4):572-585 . PubMed
  36. Zegarra‐Ruiz DF et al. 2018. Cell host & microbe. 25(1):113-127 . PubMed
  37. Kojima K, et al. 2011. Am J Respir Crit Care Med. 184:328. PubMed
  38. Riedel SS, et al. 2021. Molecular Cell. 81(11):2332-2348.e9. PubMed
  39. Rossnagl S, et al. 2016. PLoS Biol. 14: 1002562. PubMed
  40. Baptista M, et al. 2016. Nat Commun. 7:12175. PubMed
  41. Nakagawa MM, et al. 2018. Cell Rep. 25:2094. PubMed
  42. Li J, et al. 2013. J Immunol. 190:597. PubMed
  43. Wünsche P et al. 2018. Cell stem cell. 23(1):132-146 . PubMed
  44. Hohsfield LA, et al. 2021. Elife. 10:. PubMed
  45. Giotopoulos G, et al. 2015. J Exp Med. 212: 1551-1569. PubMed
RRID
AB_312806 (BioLegend Cat. No. 101307)
AB_312806 (BioLegend Cat. No. 101308)

Antigen Details

Structure
Ig superfamily, 40-60 kD
Distribution

B cells, monocyte/macrophages, NK cells, neutrophils, mast cells, dendritic cells

Function
Low affinity receptors for IgG
Ligand/Receptor
IgG
Cell Type
B cells, Dendritic cells, Macrophages, Mast cells, Monocytes, Neutrophils, NK cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Unkeless JC. 1989. J. Clin. Invest. 83:355.
3. Qiu WQ, et al. 1990. Science 248:732.

Gene ID
14130 View all products for this Gene ID 14131 View all products for this Gene ID
UniProt
View information about CD16/32 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 01.23.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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