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PE anti-mouse CD256 (APRIL) Antibody

Pricing & Availability
Clone
A3D8 (See other available formats)
Regulatory Status
RUO
Other Names
TNFSF13, TALL2, TRDL1
Isotype
Armenian Hamster IgG1, κ
Ave. Rating
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Product Citations
publications
A_A3D8_PE_012610
Intracellular staining of the murine lymphoblastic cell line EL4 (black open histogram) and EL-4 cells transfected with mouse APRIL (orange filled) with A3D8 PE.
  • A_A3D8_PE_012610
    Intracellular staining of the murine lymphoblastic cell line EL4 (black open histogram) and EL-4 cells transfected with mouse APRIL (orange filled) with A3D8 PE.
  • B_A3D8_PE_012610
    Intracellular staining of the murine macrophage like cell line P388D1 with A3D8 PE
See PE spectral data
Cat # Size Price Quantity Check Availability Save
136703 50 µg 129 CHF
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136704 200 µg 360 CHF
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Description

CD256 (APRIL, TNFSF13, TALL2, TRDL1) a transmembranal protein of 26 kD, forms a homotrimeric molecule that is cleaved from the cell surface resulting in the soluble form of APRIL; it also forms heterotrimers with BLyS. Its receptor are TNFRSF13b/TACI and TNFRSF17/BCMA. Expressed by T cells, B cells, dendritic cells, monocytes, and macrophages, APRIL induces proliferation of T and B cells, promotes IgA class switching, downregulates T-cell dependent antibody responses, and regulates the growth of a variety of tumor cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Fusion protein of murine Maltose binding protein (MBP) and soluble April
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Moreau J, et al. 2015. Blood. 126: 1184-1192. PubMed
  2. Wang W, et al. 2014. J Immunol. 192:3011. PubMed
RRID
AB_2206542 (BioLegend Cat. No. 136703)
AB_2206542 (BioLegend Cat. No. 136704)

Antigen Details

Structure
Transmembranal protein of 26 kD, forms a homotrimer that is cleaved from the cell surface resulting in the soluble form. forms heterotrimers with BLyS
Distribution

T cells, B cells, dendritic cells, monocytes, and macrophages

Function
Induces proliferation of T and B cells, promotes IgA class switching and downregulates T-cell dependent antibody responses, regulates the growth of a variety of tumor cells.
Ligand/Receptor
TNFRSF13b/TACI, TNFRSF17/BCMA
Bioactivity
Proliferation, apoptosis inhibition, IgA class switch.
Cell Type
B cells, Dendritic cells, Macrophages, Monocytes, T cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References

1. Belnoue E, et al. 2008 Blood 111:2755.
2. Xie P, et al. 2007. Immunity 27:253.
3. Mohr E, et al. 2009. J. Immunol. 182:2113.
4. Tezuka H, et al. 2007. Nature 448:92.
5. Benson MJ, et al. 2008. J. Immunol. 180:3655.

Gene ID
69583 View all products for this Gene ID
UniProt
View information about CD256 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 6    Revision Date: 06.14.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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