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PE anti-mouse CD276 (B7-H3) Antibody

Pricing & Availability
Clone
RTAA15 (See other available formats)
Regulatory Status
RUO
Other Names
B7H3, B7RP-2
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
RTAA15_PE_011909.jpg
Thioglycolate-elicited BALB/c mouse peritoneal macrophages stimulated with LPS for 24 hours and then stained with RTAA15 PE
  • RTAA15_PE_011909.jpg
    Thioglycolate-elicited BALB/c mouse peritoneal macrophages stimulated with LPS for 24 hours and then stained with RTAA15 PE
See PE spectral data
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124507 25 µg 92 CHF
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124508 100 µg 275 CHF
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Description

B7-H3 is a member of the B7 gene family. It has 88% amino acid sequence identity with human B7-H3. It is mostly expressed on professional APCs, including B cells, macrophages, and dendritic cells. Its expression on dendritic cells appears to be up-regulated by LPS. B7-H3 protein inhibits T cell activation and cytokine production. It was reported that monoclonal antibody against B7-H3 enhanced T cell proliferation in vitro and led to exacerbated EAE in vivo. Therefore, B7-H3 may be a negative regulator on T cell activation and function.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Xu J, et al. 2006. Cellular and Molecular Immunology. 3(3):235
  2. Prasad D.V.R. 2004. J.Immunology.173:2500
Product Citations
  1. Li H, et al. 2022. Cancer Cell. 40:36. PubMed
  2. Chen L, et al. 2020. Cancer Immunol Res. 0.457638889. PubMed
  3. Wolock SL, et al. 2019. Cell Rep. 28:302. PubMed
  4. Knox T, et al. 2019. Sci Rep. 9:6136. PubMed
  5. O'Hara J, et al. 2012. Infect Immun. 80:1563. PubMed
RRID
AB_1279206 (BioLegend Cat. No. 124507)
AB_1279206 (BioLegend Cat. No. 124508)

Antigen Details

Structure
About 49.7 kd transmembrane protein characterized by extracellular IgV and IgC domains.
Distribution

Mouse B7-H3 is expressed on professional APCs, including B cells, dendritic cells, and macrophages. It is reported to express on a minor fraction of CD4+ and CD8+ cells.

Ligand/Receptor
The receptor has not been identified, but it seems to be expressed on activated T cells.
Bioactivity
Mouse B7-H3 is a negative regulator of T cell activation and IL-2 production.
Cell Type
Antigen-presenting cells, Dendritic cells
Biology Area
Immunology, Costimulatory Molecules
Molecular Family
CD Molecules
Antigen References

1. Sun M et al. 2002. J. Immunology. 168:6294
2. Xu J et al. 2006. Cellular and Molecular Immunology. 3(3):235

Gene ID
102657 View all products for this Gene ID
UniProt
View information about CD276 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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